Mass spectrometry label-free quantitative analysis of proteins

被引：0

作者：

Kopylov A.T. ^{[1
]}

Zgoda V.G. ^{[1
]}

Archakov A.I. ^{[1
]}

机构：

[1] Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow 119121

来源：

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | 2010年 / 4卷 / 1期

基金：

俄罗斯基础研究基金会;

关键词：

Label-free method; Linear correlation; Mass-spectrometry; Quantitative proteomics;

D O I：

10.1134/S1990750810010075

中图分类号：

学科分类号：

摘要：

The dependence of the intensity of a mass spectrometric signal on protein concentration has been investigated using individual purified proteins and complex protein mixtures. Linearity of this dependence has been determined within the concentration ranges from 1 nM to 1 μM. In this range the dependence of the mass spectrometric signal intensity on concentration possesses a slope (tangent) characteristic for each protein studied. The efficacy of the method has been evaluated using the other methods (AQUA and 18O- labelling) employed in quantitative proteomics. © 2010 Pleiades Publishing, Ltd.

引用

页码：49 / 58

页数：9

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