Mass spectrometry label-free quantitative analysis of proteins

被引：0
|
作者：
Kopylov A.T. [1 ]
Zgoda V.G. [1 ]
Archakov A.I. [1 ]
机构：
[1] Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow 119121
来源：
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | 2010年 / 4卷 / 1期
基金：
俄罗斯基础研究基金会;
关键词：
Label-free method; Linear correlation; Mass-spectrometry; Quantitative proteomics;
D O I：
10.1134/S1990750810010075
中图分类号：
学科分类号：
摘要：
The dependence of the intensity of a mass spectrometric signal on protein concentration has been investigated using individual purified proteins and complex protein mixtures. Linearity of this dependence has been determined within the concentration ranges from 1 nM to 1 μM. In this range the dependence of the mass spectrometric signal intensity on concentration possesses a slope (tangent) characteristic for each protein studied. The efficacy of the method has been evaluated using the other methods (AQUA and 18O- labelling) employed in quantitative proteomics. © 2010 Pleiades Publishing, Ltd.
引用
收藏
页码：49 / 58
页数：9
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