Transverse relaxation optimized HCN experiment for nucleic acids: Combining the advantages of TROSY and MQ spin evolution

被引：0
作者：
Bernhard Brutscher
Jean-Pierre Simorre
机构：
[1] Jean-Pierre Ebel C.N.R.S.-C.E.A.,Institut de Biologie Structurale
来源：
Journal of Biomolecular NMR | 2001年 / 21卷
关键词：
HCN; multiple quantum coherence; NMR assignment; nucleic acids; triple resonance; TROSY;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A three-dimensional MQ-TROSY-HCN pulse sequence is presented which provides intra-base and sugar-to-base correlations for 13C, 15N labeled nucleic acids (RNA, DNA). The experiment simultaneously exploits the favorable relaxation properties of 1H-13C multiple quantum coherence for sugar carbons and of 13C TROSY-type spin evolution for base carbons. MQ-TROSY-HCN thus combines the advantages of MQ-HCN for sugar-to-base and TROSY-HCN for intra-base correlations in a single experiment. In addition, two slightly different implementations of the MQ-TROSY-HCN experiment ensure optimal performance for small and larger oligonucleotides, respectively. The advantages of the MQ-TROSY-HCN experiment compared to the best previous implementations of HCN are demonstrated for a 33 nucleotide RNA aptamer.
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页码：367 / 372
页数：5
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