Unexpected behavior of digoxin at elevated temperatures in reversed-phase liquid chromatography

Increased retention of digoxin has been observed at elevated temperatures on both 10 nm and 30 nm porediamter, RP-18 packing. This result is the opposite effect compared with the decreased retention under the same conditions with digoxin aglycon-digoxigenin. Rotation around the C−C σ-bonds in the digoxin molecule is presumed; the rod-like molecules of the newlyobtained digoxin penetrate stationary phase pores more easily thus increasing retention.