Determination of Three Nonsteroidal Anti-Inflammatory Drugs in Human Plasma by LC Coupled with Chemiluminescence Detection

被引:1
作者
Xunyu Xiong
Qunzheng Zhang
Fengmei Xiong
Yuhai Tang
机构
[1] Xi’an Shiyou University,College of Chemistry and Chemical Engineering
[2] Xi’an Jiaotong University,Institute of Analytical Science
来源
Chromatographia | 2008年 / 67卷
关键词
Column liquid chromatography; Chemiluminescence detection; Ibuprofen, naproxen and fenbufen;
D O I
暂无
中图分类号
学科分类号
摘要
A simple and sensitive method was developed for the determination of three nonsteroidal anti-inflammatory drugs (NSAIDs)—ibuprofen, naproxen and fenbufen in human plasma. The method involved in column liquid chromatographic separation and chemilumenescence (CL) detection based on the CL reaction of NSAIDs, potassium permanganate (KMnO4) and sodium sulfite (Na2SO3) in sulfuric acid (H2SO4) medium. The chromatographic separation was carried out using a reversed-phase C18 column, which allowed the selective determination of the three medicines in the complicated samples. The special features of the CL detector provided lower LOD for determination than that of existing chromatographic alternatives. The results indicated that the linear ranges were 0.01–10.0 μg mL−1 for ibuprofen, 0.001–1.0 μg mL−1 for naproxen, and 0.01–10.0 μg mL−1 for fenbufen. The limits of detection were 0.5 ng mL−1 for ibuprofen, 0.05 ng mL−1 for naproxen and 0.5 ng mL−1 for fenbufen (S/N = 3). All average recoveries were in the range of 90.0–102.3%. Finally, the method had been satisfactorily applied for the determination of ibuprofen, naproxen and fenbufen in human plasma samples.
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页码:929 / 934
页数:5
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