The cross-talk between the urokinase receptor and fMLP receptors regulates the activity of the CXCR4 chemokine receptor

被引：0

作者：

Nunzia Montuori

Katia Bifulco

Maria Vincenza Carriero

Claudio La Penna

Valeria Visconte

Daniela Alfano

Ada Pesapane

Francesca Wanda Rossi

Salvatore Salzano

Guido Rossi

Pia Ragno

机构：

[1] “Federico II” University,Department of Cellular and Molecular Biology and Pathology

[2] National Cancer Institute,Department of Experimental Oncology

[3] University of Salerno,Department of Chemistry

[4] “Federico II” University,Department of Clinical Immunology and Allergy

[5] Institute of Experimental Endocrinology and Oncology (National Research Council),undefined

来源：

Cellular and Molecular Life Sciences | 2011年 / 68卷

关键词：

uPAR; Urokinase-receptor; fMLP receptors; CXCR4; Prostate carcinoma cells;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The receptor (CXCR4) for the stromal-derived factor-1 (SDF1) and the urokinase-receptor (uPAR) are up-regulated in various tumors. We show that CXCR4-transfected cells migrate toward SDF1 on collagen (CG) and do not on vitronectin (VN). Co-expression of cell-surface uPAR, which is a VN receptor, impairs SDF1-induced migration on CG and allows migration on VN. Blocking fMLP receptors (fMLP-R), alpha-v integrins or the uPAR region capable to interact with fMLP-Rs, impairs migration of uPAR/CXCR4-transfected cells on VN and restores their migration on CG. uPAR co-expression also reduces the adherence of CXCR4-expressing cells to various components of the extracellular matrix (ECM) and influences the partitioning of beta1 and alpha-v integrins to membrane lipid-rafts, affecting ECM-dependent signaling. uPAR interference in CXCR4 activity has been confirmed in cells from prostate carcinoma. Our results demonstrate that uPAR expression regulates the adhesive and migratory ability of CXCR4-expressing cells through a mechanism involving fMLP receptors and alpha-v integrins.

引用

页码：2453 / 2467

页数：14

共 257 条

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