Electrochemical sensing of hydrogen peroxide using a glassy carbon electrode modified with multiwalled carbon nanotubes and zein nanoparticle composites: application to HepG2 cancer cell detection

被引：0
作者：
Hamed Tavakkoli
Morteza Akhond
Gholam Abbas Ghorbankhani
Ghodratollah Absalan
机构：
[1] Shiraz University,Department of Chemistry, College of Sciences
[2] Shiraz University,Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine
来源：
Microchimica Acta | 2020年 / 187卷
关键词：
Electrochemical sensor; Nanocomposite; Enzyme-free; Carbon nanotubes; Protein based biopolymers; Cancer detection;
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摘要：
A nanobiocomposite was prepared from multiwalled carbon nanotubes and zein nanoparticles. It was dispersed in water/ethanol and drop cast onto a glassy carbon electrode. The modified electrode can be used for electroreduction of H2O2 (typically at a working potential of −0.71 V vs. Ag/AgCl). The electrochemical properties of the electrode were investigated by cyclic voltammetry, linear sweep voltammetry, chronoamperometry and electrochemical impedance spectroscopy. Response to H2O2 is linear in the 0.049 to 22 μM concentration range, and the detection limit is 35 nM at pH 7.0. The sensor was successfully utilized for the measurement of H2O2 in a synthetic urine sample, and for monitoring the release of H2O2 from human dermal fibroblasts and human hepatocellular carcinoma cells.
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