A New Method for Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography

被引：0
|
作者：
O. Ozensoy
O. Arslan
S. Oznur Sinan
机构：
[1] Balikesir University,Science and Art Faculty, Department of Chemistry/Biochemistry Section
[2] Balikesir University,Science and Art Faculty, Department of Biology/Biochemistry Section
来源：
Biochemistry (Moscow) | 2004年 / 69卷
关键词：
EUPERGIT; C-250L; carbonic anhydrase; affinity chromatography;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGITR C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes.
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页码：216 / 219
页数：3
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