Update on comparative genome mapping between Malus and Pyrus

被引：40
作者：
Celton J.-M. [1 ,2 ]
Chagné D. [2 ]
Tustin S.D. [3 ]
Terakami S. [4 ]
Nishitani C. [4 ]
Yamamoto T. [4 ]
Gardiner S.E. [2 ]
机构：
[1] University of Western Cape, Biotechnology Department, Bellville, Cape Town 7535, Modderdam Road
[2] New Zealand Institute for Plant and Food Research Limited
[3] New Zealand Institute for Plant and Food Research Limited, Havelock North 4157, Hawke's Bay
[4] National Institute of Fruit Tree Science, Tsukuba
来源：
BMC Research Notes | / 2卷 / 1期
关键词：
Amplify Fragment Length Polymorphism; Simple Sequence Repeat Marker; Simple Sequence Repeat Locus; Amplify Fragment Length Polymorphism; Apple Rootstock;
D O I：
10.1186/1756-0500-2-182
中图分类号：
学科分类号：
摘要：
Background. Comparative genome mapping determines the linkage between homologous genes of related taxa. It has already been used in plants to characterize agronomically important genes in lesser studied species, using information from better studied species. In the Maloideae sub-family, which includes fruit species such as apple, pear, loquat and quince, genome co-linearity has been suggested between the genera Malus and Pyrus; however map comparisons are incomplete to date. Findings. Genetic maps for the apple rootstocks 'Malling 9' ('M.9') (Malus × domestica) and 'Robusta 5' ('R5') (Malus × robusta), and pear cultivars 'Bartlett' and 'La France' (Pyrus communis) were constructed using Simple Sequence Repeat (SSR) markers developed from both species, including a new set of 73 pear Expressed Sequence Tag (EST) SSR markers. Integrated genetic maps for apple and pear were then constructed using 87 and 131 SSR markers in common, respectively. The genetic maps were aligned using 102 markers in common, including 64 pear SSR markers and 38 apple SSR markers. Of these 102 markers, 90 anchor markers showed complete co-linearity between the two genomes. Conclusion. Our alignment of the genetic maps of two Malus cultivars of differing species origin with two Pyrus communis cultivars confirms the ready transferability of SSR markers from one genus to the other and supports a high level of co-linearity within the sub-family Maloideae between the genomes of Malus and Pyrus. © 2009 Gardiner et al; licensee BioMed Central Ltd.
引用
收藏
相关论文
共 20 条
  • [1] Bonierbale M.W., Plaisted R.L., Tanksley S.D., RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato, Genetics, 120, 4, pp. 1095-1103, (1988)
  • [2] Yamamoto T., Kimura T., Saito T., Kotobuki K., Matsuta N., Liebhard R., Gessler C., De W.E.V., Hayashi T., Genetic linkage maps of Japanese and European pears aligned to the apple consensus map, Acta Horticulturae, 663, pp. 51-56, (2004)
  • [3] Gardiner S.E., Bus V.G.M., Rusholme R.L., Chagne D., Rikkerink E.H.A., Genome mapping and molecular breeding in plants, Fruit and Nuts, 4, pp. 1-62, (2007)
  • [4] Itai A., Genome mapping and molecular breeding in plants, Fruit and Nuts, 4, pp. 157-170, (2007)
  • [5] Tatum T.C., Stepanovic S., Biradar D.P., Rayburn A.L., Korban S.S., Variation in nuclear DNA content in Malus species and cultivated apples, Genome, 48, pp. 924-930, (2005)
  • [6] Arumanagathan K., Earle E.D., Nuclear DNA content of some important plant species, Plant Mol Biol Rep, 9, pp. 229-241, (1991)
  • [7] Wolfe J.A., Wehr W., Rosaceous Chamaebatiaria -like foliage from the paleogene of western North America, Aliso, 12, pp. 177-200, (1988)
  • [8] Liebhard R., Koller B., Gianfranceschi L., Gessler C., Creating a saturated reference map for the apple (Malus × domestica Borkh.) genome, Theor Appl Genet, 106, pp. 1497-1508, (2003)
  • [9] Yamamoto T., Kimura T., Terakami S., Nishitani C., Sawamura Y., Saito T., Kotobuki K., Hayashi T., Integrated genetic linkage maps for pear based on SSR and AFLP markers, Breeding Science, 57, pp. 321-329, (2007)
  • [10] Pierantoni L., Cho K.-H., Shin I.-S., Chiodini R., Tartarini S., Dondini L., Kang S.-J., Sansavini S., Characterisation and transferability of apple SSRs to two European pear F1 populations, Theoretical and Applied Genetics, 109, pp. 1519-1524, (2004)
12