Mapping of Rym14Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley

被引:0
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作者
B. Ruge
A. Linz
R. Pickering
G. Proeseler
P. Greif
P. Wehling
机构
[1] Federal Centre for Breeding Research on Cultivated Plants,
[2] Institute of Agricultural Crops,undefined
[3] Rudolf-Schick-Platz 3a,undefined
[4] D-18190 Groß Lüsewitz,undefined
[5] Germany,undefined
[6] New Zealand Institute for Crop and Food Research Limited,undefined
[7] Private Bag 4704,undefined
[8] Christchurch,undefined
[9] New Zealand,undefined
[10] Federal Centre for Breeding Research on Cultivated Plants,undefined
[11] Institute of Epidemiology and Resistance Resources,undefined
[12] Aschersleben,undefined
[13] Germany,undefined
[14] Saatzuchtgesellschaft Streng's Erben GmbH and Co. KG,undefined
[15] Uffenheim,undefined
[16] Germany,undefined
来源
关键词
Soil-borne virus resistance; Introgression mapping;
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学科分类号
摘要
Hordeum bulbosum represents the secondary gene pool of barley and constitutes a potential source of various disease resistances in barley breeding. Interspecific crosses of H. vulgare × H. bulbosum resulted in recombinant diploid-barley progeny with immunity to BaMMV after mechanical inoculation. Tests on fields contaminated with different viruses demonstrated that resistance was effective against all European viruses of the soil-borne virus complex (BaMMV, BaYMV-1, -2). Genetic analysis revealed that resistance was dominantly inherited. Marker analysis in a F5 mapping family was performed to map the introgression in the barley genome and to estimate its size after several rounds of recombination. RFLP anchor-marker alleles indicative of an H. bulbosum introgression were found to cover an interval 2.9 cM in length on chromosome 6HS. The soil-borne virus resistance locus harboured by this introgressed segment was designated Rym14Hb. For marker-assisted selection of Rym14Hb carriers, a diagnostic codominant STS marker was derived from an AFLP fragment amplified from leaf cDNA of homozygous-resistant genotypes inoculated with BaMMV.
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页码:965 / 971
页数:6
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