Isolation and purification of Mn-peroxidase from Azospirillum brasilense SP245

被引：0
|
作者：
M. A. Kupryashina
N. Yu. Selivanov
V. E. Nikitina
机构：
[1] Russian Academy of Sciences,Institute of Biochemistry and Physiology of Plants and Microorganisms
来源：
Applied Biochemistry and Microbiology | 2012年 / 48卷
关键词：
Apply Biochemistry; Azospirillum; Bacillus Pumilus; Pyrocatechol; Syringaldazine;
D O I：
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学科分类号：
摘要：
Homogenous Mn-peroxidase of a 26-fold purity grade was isolated from a culture of Azospirillum brasilense Sp245 cultivated on a medium containing 0.1 mM pyrocatechol. The molecular weight of the enzyme is 43 kD as revealed by electrophoresis in SDS-PAAG. It was shown that the use of pyrocatechol and 2,2′-azino-bis(3-ethylbenzotiazoline-6-sulfonate) at concentrations of 0.1 and 1 mM as inductors increased the Mn-peroxidase activity by a factor of 3.
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页码：17 / 20
页数：3
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