A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood

被引：0
|
作者：
Aishun Jin
Tatsuhiko Ozawa
Kazuto Tajiri
Tsutomu Obata
Sachiko Kondo
Koshi Kinoshita
Shinichi Kadowaki
Kazuo Takahashi
Toshiro Sugiyama
Hiroyuki Kishi
Atsushi Muraguchi
机构：
[1] Graduate School of Medicine and Pharmaceutical Sciences,Department of Immunology
[2] University of Toyama,The Third Department of Internal Medicine
[3] SC World Inc.,Department of Infectious Diseases
[4] Graduate School of Medicine and Pharmaceutical Sciences,undefined
[5] University of Toyama,undefined
[6] Central Research Institute,undefined
[7] Toyama Industrial Technology Center,undefined
[8] Osaka Prefectural Institute of Public Health,undefined
来源：
Nature Medicine | 2009年 / 15卷
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摘要：
Jin and colleagues introduce a new chip-based system for the rapid identification and isolation of single antigen-specific antibody–secreting cells from human peripheral blood lymphocytes. The approach can be used to detect antibody-secreting cells for multiple antigens on the same chip and should have advantages over current technologies for isolating and producing human monoclonal antibodies of clinical significance.
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页码：1088 / 1092
页数：4
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