Linum usitatissimum (linseed/flaxseed) fixed oil: Antimicrobial activity and efficacy in bovine mastitis

被引：52
作者：
Kaithwas G. [1 ]
Mukerjee A. [1 ]
Kumar P. [2 ]
Majumdar D.K. [3 ]
机构：
[1] Department of Pharmaceutical Sciences, F.H.M.S, Allahabad Agricultural Institute-Deemed University, Allahabad
[2] Department of Veterinary Sciences, Allahabad Agricultural Institute, Deemed University, Allahabad
[3] Department of Pharmaceutics, Delhi Institute of Pharmaceutical Sciences and Research, Formerly College of Pharmacy (University of Delhi), New Delhi 110017, Pushp Vihar, Sector III
来源：
Inflammopharmacology | 2011年 / 19卷 / 1期
关键词：
Antimicrobial; CMT; Flaxseed; Linseed; Mastitis; Oil; SCC;
D O I：
10.1007/s10787-010-0047-3
中图分类号：
学科分类号：
摘要：
Purpose: In vitro antimicrobial activity and in vivo therapeutic efficacy of L. usitatissimum (linseed/flaxseed) fixed oil in bovine mastitis were investigated. Method: In vitro antimicrobial activity of L. usitatissimum fixed oil was evaluated against a number of microorganisms by disc diffusion method and MIC determination. The in vivo efficacy of the oil was evaluated in nine mastitis-affected cows divided into three groups (three in each group), following once-a-day intramammary infusion of oil, cefoperazone or an oil-cefoperazone combination for 7 days and by monitoring the California mastitis test score, somatic cell count and microbial count in milk samples. Results: The in vitro antimicrobial activity of the oil against Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli was comparable to that of cefoperazone while the antimicrobial activity against Enterococcus faecalis, Micrococcus luteus and Candida albicans, was greater than that of cefoperazone. In the in vivo study, the oil exhibited significant reduction in the California mastitis test score and somatic cell count in milk samples from infected udders following 7 days of intramammary administration suggesting its anti-inflammatory effect. The microbial count in milk samples was also reduced significantly following oil treatment. The effects were comparable to the treatment by cefoperazone (Mastiwock) alone or in combination with the oil. Apparently, the anti-inflammatory and antimicrobial properties of the oil contribute to its therapeutic efficacy in mastitis; the oil could be used as an alternative treatment for bovine mastitis Conclusion: The results suggest possible therapeutic potential of L. usitatissimum fixed oil in bovine mastitis. © 2010 Springer Basel AG.
引用
收藏
页码：45 / 52
页数：7
相关论文
共 27 条
  • [1] Delignette-Muller M.L., Flandrois J.P., An accurate diffusion method for determining bacterial sensitivity to antibiotics, Journal of Antimicrobial Chemotherapy, 34, 1, pp. 73-81, (1994)
  • [2] Dilika F., Bremner P.D., Meyer J.J.M., Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: A plant used during circumcision rites, Fitoterapia, 71, 4, pp. 450-452, (2000)
  • [3] Freese E., Shew C.W., Galliers E., Function of lipophilic acids as antimicrobial food additives, Nature, 241, pp. 321-325, (1973)
  • [4] Greenway D.L.A., Dyke K.G.H., Mechanism of the inhibitory action of linoleic acid on the growth of Staphylococcus aureus, Journal of General Microbiology, 115, 1, pp. 233-245, (1979)
  • [5] Hardman J.G., Limbird L.E., Goodman and Gillman's the Pharmacological Basis of Therapeutics, (2001)
  • [6] Enumeration of Somatic Cells. FIL-IDF Standard No. 148 A, (1995)
  • [7] Kabara J.J., Swieczkowski D.M., Conley A.J., Truant J.P., Fatty acids and derivatives as antimicrobial agents, Antimicrob Agents Chemother, 2, 1, pp. 23-28, (1972)
  • [8] Kaithwas G., Majumdar D.K., Therapeutic effect of Linum usitatissimum (flaxseed/linseed) fixed oil on acute and chronic arthritic models in albino rats, Inflammopharmacology, 18, 3, pp. 127-136, (2010)
  • [9] Kaithwas G., Majumdar D.K., Evaluation of antiulcer and antisecretory potential of Linum usitatissimum fixed oil and possible mechanism of action, Inflammopharmacology, 18, 3, pp. 137-145, (2010)
  • [10] Kivaria F.M., Noordhuizen J.P.T.M., A retrospective study of the aetiology and temporal distribution of bovine clinical mastitis in smallholder dairy herds in the Dar es Salaam region of Tanzania, Veterinary Journal, 173, 3, pp. 617-622, (2007)
123