Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

被引：22
作者：
Bouchedja D.N. [1 ,2 ,3 ]
Danthine S. [3 ]
Kar T. [1 ]
Fickers P. [1 ]
Boudjellal A. [2 ]
Delvigne F. [1 ]
机构：
[1] Unité de Bio-Industries, Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, Gembloux
[2] INATAA, Université Frères Mentouri Constantine, Route de Aïn El Bey, Constantine
[3] Unité de science des aliments et formulation Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, Gembloux
来源：
Bioresources and Bioprocessing | / 4卷 / 1期
关键词：
Cell growth; Cells; shape; Lipid accumulation; Online flow cytometry; Yarrowia lipolytica;
D O I：
10.1186/s40643-016-0132-6
中图分类号：
学科分类号：
摘要：
This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation by Y. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors. © 2017, The Author(s).
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  • [1] Aggelis G., Komaitis M., Papanikolaou S., Papadopoulos G., A mathematical model for the study of lipid accumulation in oleaginous microorganisms. I: lipid accumulation during growth of Mucor circinelloides CBS 172-27 on a vegetable oil, Grasas Aceites, 46, pp. 169-173, (1995)
  • [2] Alonso F.O.M., Oliveira E.B.L., Dellamora-Ortiz G.M., Pereira-Meirelles F.V., Improvement of lipase production at different stirring speeds and oxygen levels, Braz J Chem Eng, 22, pp. 9-18, (2005)
  • [3] Barth G., Gaillardin C., Physiology and genetics of the dimorphic fungus Yarrowia lipolytica, FEMS Microbiol Rev, 19, pp. 219-237, (1997)
  • [4] Beopoulos A., Mrozova Z., Thevenieau F., Le Dall M.T., Hapala I., Papanikolaou S., Chardot T., Nicaud J.M., Control of lipid accumulation in the yeast Yarrowia lipolytica, Appl Environ Microbiol, 74, pp. 7779-7789, (2008)
  • [5] Beopoulos A., Chardot T., Nicaud J.-M., Yarrowia lipolytica: a model and a tool to understand the mechanisms implicated in lipid accumulation, Biochimie, 91, pp. 692-696, (2009)
  • [6] Beopoulos A., Nicaud J.-M., Gaillardin C., An overview of lipid metabolism in yeasts and its impact on biotechnological processes, Appl Microbiol Biotechnol, 90, pp. 1193-1206, (2011)
  • [7] Bradner J.R., Nevalainen K.M.H., Metabolic activity in filamentous fungi can be analysed by flow cytometry, J Microbiol Methods, 54, pp. 193-201, (2003)
  • [8] Cervantes-Chavez J.A., Kronberg F., Passeron S., Ruiz-Herrera J., Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica, Fungal Genet Biol, 46, pp. 390-399, (2009)
  • [9] Chen D.-C., Beckerich J.-M., Gaillardin C., One-step transformation of the dimorphic yeast Yarrowia lipolytica, Appl Microbiol Biotechnol, 48, pp. 232-235, (1997)
  • [10] Coelho M., Amaral P., Belo I., Yarrowia lipolytica: an industrial workhorse, Curr Res Technol Educ Top Appl Microbiol Microbial Biotechnol, 2, 2010, pp. 930-994, (2010)
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