Immobilization of Alcaligenes faecalis penicillin G acylase on epoxy-type supports

被引：0

作者：

J. Sun

Y. Zhou

Z. Yuan

G. Xu

机构：

[1] Chinese Academy of Sciences,Dalian Institute of Chemical Physics

[2] East China University of Science and Technology,State Key Laboratory of Bioreactor Engineering

[3] Chinese Academy of Sciences,Institute of Biochemistry and Cell Biology, Shanghai Institutes for Life Sciences

来源：

Applied Biochemistry and Microbiology | 2009年 / 45卷

关键词：

Immobilization; Apply Biochemistry; Immobilize Enzyme; Phenyl Acetic Acid; Glycidyl Methacrylate;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Alcaligenes faecalis penicillin G acylase has several desired features over other penicillin G acylases and its use in industry requires immobilization. In this work, two novel supports ZH-EP (epoxy type) and ZH-HA (epoxy-amino type) were used to immobilize Alcaligenes faecalis penicillin G acylase (AfPGA) with Eupergit C as reference. The saturation of immobilized protein on ZH-EP (269 mg/g, 116 h) and ZH-HA (296 mg/g, 15 h) was obtained more rapidly than Eupergit C (197 mg/g, 260 h). And the activity of immobilized AfPGA on ZH-EP (520 U/g) and ZH-HA (2200 U/g) was higher than that on Eupergit C (310 U/g). The properties of three immobilized enzymes were compared and no obvious difference was observed, which indicated that ZH-EP and ZH-HA were promised in industry.

引用

页码：478 / 483

页数：5

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