Effects of Andrographitis Paniculata extracts on the expression of CD40 in endothelial cells

被引：0
作者：
Jie Zhang
Shusheng Li
Lei Wan
机构：
[1] Huazhong University of Science and Technology,Department of Emergency, Tongji Hospital, Tongji Medical College
来源：
Journal of Huazhong University of Science and Technology | 2007年 / 27卷
关键词：
API; endothelial cells; CD40; atherosclerosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In order to investigate the expression of CD40 in endothelial cells (ECs) in a variety of injured conditions and the interventional role of Andrographitis Paniculata isolate (API0134), the thoracic aorta ECs of guinea pigs were cultured in vitro until the third passage, incubated in the presence of media containing xanthine oxidase (XO) and xanthine (Xan) which produced oxygen free radical (OFR group); oxidized-LDL (ox-LDL group); XO, Xan and API0134 (OFR+API0134 group); or ox-LDL and API0134 (ox-LDL+API0134 group). The expression of CD40 in ECs was detected by immunofluorescence assay and reverse transcription-PCR (RT-PCR). The results showed as compared with the control group, the expression of CD40 in ECs in OFR group and ox-LDL group was increased (P<0.01), but attenuated significantly in OFR+ API0134 group and ox-LDL+API0134 group (P<0.05). It was suggested that API0134 could protect atherosclerosis by inhibiting the expression of CD40 molecule in injured ECs.
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页码：138 / 141
页数：3
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