Discussion of the AED workshop.

被引：0
作者：
Zafar Iqbal
J.M. Midgley
D.G. Watson
S.D. Karditsas
G.N. Dutton
W. Wilson
机构：
[1] University of Strathclyde,Department of Pharmacy
[2] Tennent Institute of Ophthalmology,undefined
[3] Western Infirmary,undefined
来源：
Pharmacy World and Science | 1997年 / 19卷 / 5期
关键词：
Ascorbic acid; Depolymerization; Eye; High performance gel permeation chromatography; Hyaluronic acid; Molecular weight;
D O I：
10.1023/A:1008644400293
中图分类号：
学科分类号：
摘要：
A method has been developed for the determination of the molecular weight of hyaluronic acid by high performance gel permeation chromatography. The molecular weight distribution was determined using three polymeric columns. Columns were calibrated using Pullulan Polymer molecular weight standards. The average molecular weight (± SD) of the hyaluronic acid in bovine vitreous humour and Healon® were 1.20 ¥ 106 Da (± 0.49 ¥ 106) and 3.01 ¥ 106 Da (± 0.14 ¥ 106), respectively. Furthermore, the depolymerization of the hyaluronic acid in the presence of ascorbic acid (the concentration found in human eye) was studied. Samples incubated in the presence of atmospheric air showed more depolymerization than those where atmospheric air had been flushed-out with nitrogen.
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页码：246 / 250
页数：4
相关论文
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