High-throughput sequencing analysis reveals the genetic diversity of different regions of the murine norovirus genome during in vitro replication

被引：0
作者：
Axel Mauroy
Bernard Taminiau
Carine Nezer
Elsa Ghurburrun
Denis Baurain
Georges Daube
Etienne Thiry
机构：
[1] University of Liège,Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine
[2] University of Liège,Food Microbiology, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine
[3] Quality Partner S.A.,Eukaryotic Phylogenomics, Department of Life Sciences
[4] University of Liège,undefined
来源：
Archives of Virology | 2017年 / 162卷
关键词：
Norovirus; Murine; Next-generation sequencing; Genetic diversity; Mutation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, we report the genetic diversity and nucleotide mutation rates of five representative regions of the murine norovirus genome during in vitro passages. The mutation rates were similar in genomic regions encompassing partial coding sequences for non-structural (NS) 1-2, NS5, NS6, NS7 proteins within open reading frame (ORF) 1. In a region encoding a portion of the major capsid protein (VP1) within ORF2 (also including the ORF4 region) and a portion of the minor structural protein (VP2), the mutation rates were estimated to be at least one order of magnitude higher. The VP2 coding region was found to have the highest mutation rate.
引用
收藏
页码：1019 / 1023
页数：4
相关论文
共 90 条
[1]  
Karst SM(2014)Advances in norovirus biology Cell Host Microbe 15 668-680
[2]  
Wobus CE(2014)Norovirus gene expression and replication J Gen Virol 95 278-291
[3]  
Goodfellow IG(2015)ADARs and the balance game between virus infection and innate immune cell response Curr Issues Mol Biol 17 37-51
[4]  
Thorne LG(2013)The role of mutational robustness in RNA virus evolution Nat Rev Microbiol 11 327-336
[5]  
Goodfellow IG(2012)From molecular genetics to phylodynamics: evolutionary relevance of mutation rates across viruses PLoS Pathog 8 e1002685-12138
[6]  
Tomaselli S(2009)Origin and phylodynamics of rabbit hemorrhagic disease virus J Virol 83 12129-49
[7]  
Galeano F(2013)Genetic and evolutionary perspectives on genogroup III, genotype 2 bovine noroviruses Arch Virol 159 39-8666
[8]  
Locatelli F(2011)Comparative evolution of GII.3 and GII.4 norovirus over a 31-year period J Virol 85 8656-11901
[9]  
Gallo A(2009)Evolutionary dynamics of GII.4 noroviruses over a 34-year period J Virol 83 11890-10473
[10]  
Lauring AS(2004)Replication of norovirus in cell culture reveals a tropism for dendritic cells and macrophages PLoS Biol 2 e432-122
123456789