Yellow pigment from gardenia fruit: structural identification and evaluation of cytotoxic activity in HepG2 cells by induction of apoptosis

被引：0
作者：
Liqin Tang
Haocheng Liu
Manqin Fu
Yujuan Xu
Jing Wen
Jijun Wu
Yuanshan Yu
Xian Lin
Lu Li
Zhibin Bu
Wanyuan Yang
机构：
[1] Guangdong Key Laboratory of Agricultural Products Processing,Sericultural & Agri
[2] Jiangxi Agricultural University,Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture
[3] Guangdong Jiabao Group Co.,College of Agronomy
[4] Ltd.,undefined
来源：
Food Science and Biotechnology | 2022年 / 31卷
关键词：
Yellow pigment; LC-TOF–MS/MS; HepG2; Crocins; Apoptosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The preparation process of yellow pigment (YP) from gardenia (Gardenia jasminoides) fruit was investigated, and the main components of YP were characterized by liquid chromatography-time of flight-mass spectrometer/mass spectrometer (LC-TOF–MS/MS). Furthermore, cytotoxic activity in HepG2 cells by induction of apoptosis was also evaluated. The preparation results indicated that the color value of YP was 498.34, which was 8.6 times higher than crude YP. Fifteen compounds in YP were identified, and crocins were the predominant compounds. The cell experiment results showed that YP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner. Moreover, YP also inhibited HepG2 cells in G2/M stage, increased the level of intracellular reactive oxygen species (ROS), and enhanced cell apoptosis. Real-time quantitative polymerase chain reaction (RT-PCR) analysis revealed the up-regulation of caspase-3, 8, 9, and bax and down-regulation of bcl-2 in HepG2 cells. Overall, these findings suggested that YP had potential cytotoxic activity in HepG2 cells by induction of apoptosis, which might be beneficial to human health.
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页码：1389 / 1399
页数：10
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