Amylase-assisted extraction and antioxidant activity of polysaccharides from Gracilaria lemaneiformis

被引：0
作者：
Shengjun Wu
Mingsheng Lu
Shujun Wang
机构：
[1] Huaihai Institute of Technology,Marine Resources Development Institute of Jiangsu
[2] Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Key Laboratory of Marine Biotechnology
[3] Huaihai Institute of Technology,College of Marine Life and Fisheries
[4] Huaihai Institute of Technology,undefined
来源：
3 Biotech | 2017年 / 7卷
关键词：
Polysaccharides; Antioxidant activity; Thermostable α-amylase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Gracilaria lemaneiformis (G. lemaneiformis) is rich in polysaccharides that have many functional activities. In this study, the thermostable α-amylase-assisted extraction and antioxidant activity of water-soluble polysaccharides from G. lemaneiformis were investigated. The extraction conditions were optimized as follows: time, 40 min; temperature, 95 °C; pH, 5 and enzyme amount, 6000 U/g, under which the yield of G. lemaneiformis polysaccharides (GPs) reached 49.15% dry base. The GPs were characterized by monosaccharide composition, FTIR spectrum, UV spectrum and antioxidant activities. The results indicate that the GPs had strong hydroxyl radical activity at a concentration of 100 μg/mL and could be used as potential antioxidants.
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