Combined Effect of Hydrogen Peroxide Induced Oxidative Stress and IL-1α on IL-8 Production in CaCo-2 Cells (a Human Colon Carcinoma Cell Line) and Normal Intestinal Epithelial Cells

Oxidative stress, IL-1α, and IL-8 are known to contribute to mucosal inflammation of the gastrointestinal tract. We examined the IL-8 response after brief exposure to hydrogen peroxide induced oxidative stress in CaCo-2 cells (a human colon carcinoma cell line) and in human intestinal epithelial cells. In addition, we examined whether exposure to oxidative stress, followed by IL-1α, could modulate IL-8 production. A transient up-regulation of IL-8 mRNA expression was observed after hydrogen peroxide treatment. Hydrogen peroxide induced oxidative stress was also observed to promote IL-8 secretion. Exposure to hydrogen peroxide, followed by IL-1α, enhanced IL-8 production over that achieved with IL-1α alone. Thus, oxidative stress and IL-1α were observed to cooperatively enhance IL-8 production.