Maturation of avocado somatic embryos and plant recovery

被引：14
作者：
Witjaksono [1 ]
Litz R.E. [1 ]
机构：
[1] Trop. Research and Education Center, University of Florida, Homestead, FL 33031-3314
来源：
Plant Cell, Tissue and Organ Culture | 1999年 / 58卷 / 2期
关键词：
Avocado; Conversion; Germination; Somatic embryo;
D O I：
10.1023/A:1006344127546
中图分类号：
学科分类号：
摘要：
Avocado proembryonic masses from suspension cultures were used to develop a protocol for somatic embryo development and maturation. Avocado somatic embryos could develop from proembryonic masses both in liquid and on semisolid medium but only the latter could develop to maturity. Size and number of opaque somatic embryos were affected by gellan gum concentration, with the optimum response obtained on medium supplemented with 6-7 g l-1 gellan gum. The optimum sucrose concentration for recovery of opaque somatic embryos was 90 g l-1; however, the development of embryos was suppressed at this concentration. Consequently, recovery of cotyledonary, opaque somatic embryos was achieved on medium with 30 g l-1 sucrose. Somatic embryo development from dedifferentiating proembryonic masses required media with a high ratio of NO3/-:NH4/+ (1:0 and 3:1) as opposed to the standard ratio (2:1) of MS medium. Germination of somatic embryos was sporadic. In order to increase the frequency of plant recovery, shoots that developed from somatic embryos were micropropagated using standard protocols.
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页码：141 / 148
页数：7
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共 20 条
  • [1] DeWald S.G., Litz R.E., Moore G.A., Maturation and germination of mango somatic embryos, J. Amer. Soc. Hort. Sci., 114, pp. 837-841, (1989)
  • [2] Etienne H., Montoro P., Michaux-Ferriere N., Carron M.P., Effect of desiccation, medium osmolarity and abscisic acid on the maturation of Hevea brasiliensis somatic embryos, J. Exp. Bot., 44, pp. 1613-1619, (1993)
  • [3] Fujiwara T., Komamine A., Effect of various growth regulators on the embryogenesis in a carrot cell suspension culture, Plant Sci. Lett., 5, pp. 359-364, (1975)
  • [4] Gamborg O.L., Miller R.A., Ojima K., Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res., 50, pp. 151-158, (1968)
  • [5] Halperin W., Wetherell D.R., Ammonium requirement for embryogenesis in vitro, Nature, 205, pp. 519-520, (1965)
  • [6] Klimaszewska K., Smith D.R., Maturation of somatic embryos of Pinus strobus is promoted by a high concentration of gellan gum, Physiol Plant, 100, pp. 949-957, (1997)
  • [7] Litz R.E., Conover R.A., In vitro somatic embryogenesis and plant regeneration from Carica papaya L. ovular callus, Plant Sci. Lett., 26, pp. 153-158, (1982)
  • [8] Monsalud M.J., Mathews H., Litz R.E., Gray D.J., Control of hyperhydricity of mango somatic embryos, Plant Cell. Tiss. Org. Cult., 42, pp. 195-206, (1995)
  • [9] Mooney P.A., Van Staden J., Induction of embryogenesis in callus from immature embryos of Persea americana, Can. J. Bot., 65, pp. 622-626, (1987)
  • [10] Murashige T., Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 15, pp. 473-497, (1962)
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