Penehyclidine hydrochloride alleviates lung ischemia-reperfusion injury by inhibiting pyroptosis

被引:3
作者
Liu, Rongfang [1 ,2 ]
Zhang, Xuguang [3 ]
Yan, Jing [4 ]
Liu, Shan [5 ]
Li, Yongle [2 ]
Wu, Guangyi [2 ]
Gao, Jingui [1 ]
机构
[1] Hebei Med Univ, Dept Anesthesiol, Hosp 2, 215 HePing West Rd, Shijiazhuang 050000, Peoples R China
[2] Hebei Univ, Affiliated Hosp, Dept Anesthesiol, Baoding 071000, Peoples R China
[3] Hebei Univ, Affiliated Hosp, Dept Thorac Surg, Baoding 071000, Peoples R China
[4] Hebei Med Univ, Electron Microscope Room, Shijiazhuang 050000, Peoples R China
[5] Hebei Univ, Affiliated Hosp, Dept Pathol, Baoding 071000, Peoples R China
关键词
Inflammatory response; Left hilum clamp; Lung ischemia-reperfusion injury; Oxidative stress; Penehyclidine hydrochloride; Pyroptosis; ISCHEMIA/REPERFUSION INJURY; DEXMEDETOMIDINE; INFLAMMATION; PROTECTS; RATS;
D O I
10.1186/s12890-024-03018-5
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Objective The aim of this research was to examine how penehyclidine hydrochloride (PHC) impacts the occurrence of pyroptosis in lung tissue cells within a rat model of lung ischemia-reperfusion injury.Methods Twenty-four Sprague Dawley (SD) rats, weighing 250 g to 270 g, were randomly distributed into three distinct groups as outlined below: a sham operation group (S group), a control group (C group), and a test group (PHC group). Rats in the PHC group received a preliminary intravenous injection of PHC at a dose of 3 mg/kg. At the conclusion of the experiment, lung tissue and blood samples were collected and properly stored for subsequent analysis. The levels of malondialdehyde, superoxide dismutase, and myeloperoxidase in the lung tissue, as well as IL-18 and IL-1 beta in the blood serum, were assessed using an Elisa kit. Pyroptosis-related proteins, including Caspase1 p20, GSDMD-N, and NLRP3, were detected through the western blot method. Additionally, the dry-to-wet ratio (D/W) of the lung tissue and the findings from the blood gas analysis were also documented.Results In contrast to the control group, the PHC group showed enhancements in oxygenation metrics, reductions in oxidative stress and inflammatory reactions, and a decrease in lung injury. Additionally, the PHC group exhibited lowered levels of pyroptosis-associated proteins, including the N-terminal segment of gasdermin D (GSDMD-N), caspase-1p20, and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3).Conclusion Pre-administration of PHC has the potential to mitigate lung ischemia-reperfusion injuries by suppressing the pyroptosis of lung tissue cells, diminishing inflammatory reactions, and enhancing lung function. The primary mechanism behind anti-pyroptotic effect of PHC appears to involve the inhibition of oxidative stress.
引用
收藏
页数:9
相关论文
共 42 条
[21]   Pyroptosis in combinatorial treatment to improve cancer patients' outcome, is that what we want? [J].
Pezuk, Julia Alejandra .
EBIOMEDICINE, 2019, 41 :17-18
[22]   Pyroptosis in inflammatory diseases and cancer [J].
Rao, Zhiping ;
Zhu, Yutong ;
Yang, Peng ;
Chen, Zhuang ;
Xia, Yuqiong ;
Qiao, Chaoqiang ;
Liu, Weijing ;
Deng, Hongzhang ;
Li, Jianxiong ;
Ning, Pengbo ;
Wang, Zhongliang .
THERANOSTICS, 2022, 12 (09) :4310-4329
[23]   Pirfenidone alleviates lung ischemia-reperfusion injury in a rat model [J].
Saito, Masao ;
Chen-Yoshikawa, Toyofumi F. ;
Suetsugu, Kimitaka ;
Okabe, Ryo ;
Takahagi, Akihiro ;
Masuda, Satohiro ;
Date, Hiroshi .
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2019, 158 (01) :289-296
[24]   Neuroprotective effects of penehyclidine hydrochloride against cerebral ischemia/reperfusion injury in mice [J].
Shu, Ya ;
Yang, Yin ;
Zhang, Pengbo .
BRAIN RESEARCH BULLETIN, 2016, 121 :115-123
[25]   A Selective M1 and M3 Receptor Antagonist, Penehyclidine Hydrochloride, Exerts Antidepressant-Like Effect in Mice [J].
Sun, Xiaojing ;
Sun, Congcong ;
Zhai, Lingyan ;
Dong, Wei .
NEUROCHEMICAL RESEARCH, 2019, 44 (12) :2723-2732
[26]   Ischemic Postconditioning Alleviates Cerebral Ischemia-Reperfusion Injury Through Activating Autophagy During Early Reperfusion in Rats [J].
Sun, Yameng ;
Zhang, Ting ;
Zhang, Yan ;
Li, Jinfeng ;
Jin, Lei ;
Sun, Yinyi ;
Shi, Nan ;
Liu, Kangyong ;
Sun, Xiaojiang .
NEUROCHEMICAL RESEARCH, 2018, 43 (09) :1826-1840
[27]   Protective effects of scopolamine and penehyclidine hydrochloride on acute cerebral ischemia-reperfusion injury after cardiopulmonary resuscitation and effects on cytokines [J].
Wang, Dengqin ;
Jiang, Qi ;
Du, Xiuling .
EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (02) :2027-2031
[28]  
Wang G, 2018, EUR REV MED PHARMACO, V22, P5712, DOI 10.26355/eurrev_201809_15839
[29]   Dexmedetomidine Alleviates Lung Oxidative Stress Injury Induced by Ischemia-Reperfusion in Diabetic Rats via the Nrf2-Sulfiredoxin1 Pathway [J].
Wang, Xuan ;
Zhang, Bing ;
Li, Guangqi ;
Zhao, Han ;
Tian, Xiaojun ;
Yu, Junmin ;
Yin, Yanwei ;
Meng, Chao .
BIOMED RESEARCH INTERNATIONAL, 2022, 2022
[30]   Penehyclidine hydrochloride preconditioning provides pulmonary and systemic protection in a rat model of lung ischaemia reperfusion injury [J].
Wang, Yaguang ;
Lin, Duomao ;
Tan, Hongbao ;
Gao, Yafen ;
Ma, Jun .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2018, 839 :1-11