Isolation and characterization of novel microsatellite markers in Codonopsis tangshen (Campanulaceae)

In this study, sixteen novel microsatellite loci primers were developed for codonopsis tangshen, a traditional endemic medicinal plant species in the central-western China. The polymorphism of each locus was assessed through 62 individuals from two natural populations. Number of alleles per locus ranged from 5 to 13. The observed heterozygosity (HO) ranged between 0.396 and 0.823, and the expected heterozygosity (HE) was between 0.528 and 0.931. There was no significant linkage disequilibrium was detected between pairs of loci. The markers presented in the study are sufficiently polymorphic and quite informative to investigate the genetic diversity and population structure of C. tangshen.