Thermosensitive folic acid-targeted poly (ethylene-co-vinyl alcohol) hemisuccinate polymeric nanoparticles for delivery of epirubicin to breast cancer cells

被引：0
作者：
Farshid Hassanzadeh
Sahar Maaleki
Jaleh Varshosaz
Ghadam Ali Khodarahmi
Maryam Farzan
Mahboubeh Rostami
机构：
[1] Isfahan University of Medical Sciences,Novel Drug Delivery Systems Research Centre and Department of Medicinal Chemistry, School of Pharmacy
[2] Isfahan University of Medical Sciences,Department of Medicinal Chemistry, School of Pharmacy
[3] Isfahan University of Medical Sciences,Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre
来源：
Iranian Polymer Journal | 2016年 / 25卷
关键词：
Targeted polymeric nanoparticles; Thermosensitive; EVOH; Epirubicin (EPI); Breast cancer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel thermosensitive folic acid (FA)-targeted succinylated poly (ethylene-co-vinyl alcohol) (EVOH) (EVOHS-FA) nanocarrier was synthesized for the specific delivery of epirubicin (EPI) to MCF-7 breast cancer cell line. Three different ratios of synthesized EVOH-Suc were reacted with FA. The structure of the desired products (EVOHS40-FA, EVOHS60-FA and EVOHS80-FA) was confirmed by 1H NMR and FTIR techniques. Nanoparticles were obtained by nano-precipitation procedure using DMSO/H2O as solvent/anti-solvent. The particle size, zeta potential, entrapment efficacy and in vitro release profile of the final formulations in different temperatures were measured. The optimized nanoparticles had the particle size of 214 ± 8.5 nm, zeta potential of −29.6 mV, PDI of 0.198 ± 0.04, and a high encapsulation efficiency that released the drug efficiently within 450 h at the temperature of 40 °C compared to 37 °C. The morphology of nanoparticles was studied by scanning electron microscopy. The in vitro cytotoxicity was evaluated using the MTT assay on MCF-7 cell lines in response to temperatures of 37 and 40 °C. The MTT assay indicated that the targeted nanoparticles carrying EPI were significantly more cytotoxic than the non-targeted nanoparticles and the free drug at 40 °C.
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页码：967 / 976
页数：9
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