The widespread occurrences of Beet soil borne virus and RNA-5 containing Beet necrotic yellow vein virus isolates in sugar beet production areas in Turkey

被引：0
作者：
Nazli D. Kutluk Yilmaz
Miray Arli Sokmen
Riza Kaya
Mehmet Ali Sevik
Berna Tunali
Sadık Demirtaş
机构：
[1] Ondokuz Mayis University,Faculty of Agriculture, Department of Plant Protection
[2] Sugar Institute,Department of Phytopathology
[3] Ondokuz Mayis University,Faculty of Arts and Science, Department of Biology
来源：
European Journal of Plant Pathology | 2016年 / 144卷
关键词：
RNA-5; p26; Rhizomania; Sugar beet;
D O I：
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中图分类号：
学科分类号：
摘要：
A total of 632 soil samples were collected from different sugar beet fields in 32 provinces during 2005, 2010 and 2011 growing seasons. Incidences of Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV) and their vector Polymyxa betae were determined by bait plant tests using ELISA and root staining techniques. The percentages of BNYVV and BSBV infested soil samples were 38 and 49.8 %, respectively. The study showed that P. betae was very common (87.7 %) in the sampled sugar beet fields. Additionally, the presence of BNYVV RNA-5 component was investigated by RT-PCR using the primers specific for p26 coding region. Out of 240 BNYVV-infected samples, 85 were selected according to their geographic origin, and 62.4 % of them belonging to 29 provinces were found to contain the RNA-5 segment. The partial nucleotide sequences of RNA-5 of BNYVV isolates from Igdir, Edirne, Ankara and Konya provinces of Turkey were obtained. Nucleotide sequencing and phylogenetic analyses showed that RNA-5 of Turkish BNYVV isolates is closer to the J type BNYVV isolates than the P type isolates. These results also indicated that BNYVV populations containing RNA-5 is highly widespread in sugar beet production areas in Turkey.
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页码：443 / 455
页数：12
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共 157 条
[21]  
Andika IB(2010)Moleculer analyses of European A, B and P type sources of Beet necrotic yellow vein virus and detection of the rare P type in Kazakhstan Journal of Plant Pathology 92 497-500
[22]  
Han C(2007)Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products Plant Patholology 56 311-316
[23]  
Tamada T(2001)Genome properties of beet virus Q, a new furo-like virus from sugar beet, determined from unpurified virus Journal of Turkish Phytopathology 30 21-25
[24]  
Clark M(2008)Distribution of various types and P25 subtypes of Virus Genes 36 429-432
[25]  
Adams AM(2009) in Germany and other European countries Bioinformatics 25 1451-1452
[26]  
Farzadfar S(2005)Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existance of two major strain groups of beet necrotic yellow vein virus Journal of General Virology 86 2115-2125
[27]  
Pourrahim R(2009)Occurrence of soilborne sugar beet viruses transmitted by Archives of Virology 154 501-506
[28]  
Golnaraghi AR(2003) northern and central parts of Turkey Applied and Environmental Microbiology 69 2356-2360
[29]  
Ahoonmanesh A(1999)Partial nucleotide sequence analysis of Turkish isolates of Archives of Virology 144 879-892
[30]  
Felsenstein J(2002) (BNYVV) RNA-3 Journal of Phytopathology 150 657-662
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