Molecular genetic characteristics of Saccharomyces cerevisiae distillers’ yeasts

被引：0
作者：
E. S. Naumova
A. Zh. Sadykova
N. N. Martynenko
G. I. Naumov
机构：
[1] Research Institute for Genetics and Selection of Industrial Microorganisms,
[2] Moscow State University of Food Production,undefined
来源：
Microbiology | 2013年 / 82卷
关键词：
distillers’ yeast; PCR-RFLP analysis of rDNA 5.8S-ITS fragment; molecular karyotyping; Southern hybridization; and ; genes; thermotolerance;
D O I：
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学科分类号：
摘要：
Genomes of 36 Saccharomyces distillers’ strains, mainly of domestic origin, were studied by PCR-RFLP analysis of rDNA 5.8S-ITS fragment, molecular karyotyping, and Southern hybridization. Molecular analysis revealed that all the strains belonged to the species S. cerevisiae. According to the karyotypic analysis, many of the strains were aneuploid. Accumulation of polymeric genes SUC and MAL was detected in S. cerevisiae distillers’ strains. Accumulation of polymeric genes of sugar fermentation may be of adaptive importance and may result in increased fermentation activity of the strains. It was demonstrated that most strains fermenting maltose after one day of fermentation possessed several MAL genes. Thermotolerant strains with high fermentation activity were selected.
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页码：175 / 185
页数：10
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