PNA-tagged Peptide Microarrays for Ratiometric Activity Detection of Cellular Protein Kinases

被引：0
作者：
Hiromu Ikeda
Yoshihiro Yayama
Akito Hata
Jumpei Kamimoto
Tatsuhiro Yamamoto
Takeshi Mori
Yoshiki Katayama
机构：
[1] Kyushu University,Department of Applied Chemistry
[2] Kyushu University,Center for Future Chemistry
[3] Information Technologies and Nanotechnologies,Institute of System
来源：
Analytical Sciences | 2014年 / 30卷
关键词：
Protein kinase; phosphorylation; microarray; PNA; peptide; IMAC; ratiometry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.
引用
收藏
页码：631 / 635
页数：4
相关论文
共 138 条
[1]  
Hunter T(2000)undefined Cell 100 113-undefined
[2]  
Manning G(2002)undefined Science 298 1912-undefined
[3]  
Whyte D B(2006)undefined Nat. Rev. Drug Discovery 5 310-undefined
[4]  
Martinez R(2008)undefined Nat. Chem. Biol. 4 132-undefined
[5]  
Hunter T(2005)undefined ChemBioChem 6 513-undefined
[6]  
Sudarsanam S(2011)undefined Proteomics 11 4595-undefined
[7]  
Kingsmore S F(2004)undefined J. Biol. Chem. 279 49206-undefined
[8]  
Krutzik P O(2008)undefined Nat. Med. 14 1236-undefined
[9]  
Crane J M(2009)undefined Cancer Res. 69 5987-undefined
[10]  
Clutter M R(2009)undefined Anal. Biochem. 387 150-undefined
12345678910