Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells

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作者
Ruifeng Yang
Ying Zheng
Michelle Burrows
Shujing Liu
Zhi Wei
Arben Nace
Wei Guo
Suresh Kumar
George Cotsarelis
Xiaowei Xu
机构
[1] Perelman School of Medicine,Department of Pathology and Laboratory Medicine
[2] University of Pennsylvania,Department of Dermatology
[3] Kligman Laboratories,Department of Computer Science
[4] Perelman School of Medicine,Department of Biology
[5] University of Pennsylvania,undefined
[6] New Jersey Institute of Technology,undefined
[7] University of Pennsylvania,undefined
来源
Nature Communications | / 5卷
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摘要
Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. The isolation and propagation of human EpSCs for tissue engineering purposes remains a challenge. Here we develop a strategy to differentiate human iPSCs (hiPSCs) into CD200+/ITGA6+ EpSCs that can reconstitute the epithelial components of the hair follicle and interfollicular epidermis. The hiPSC-derived CD200+/ITGA6+ cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. Human iPSC-derived CD200+/ITGA6+ cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. The regenerated hair follicles possess a KRT15+ stem cell population and produce hair shafts expressing hair-specific keratins. These results suggest an approach for generating large numbers of human EpSCs for tissue engineering and new treatments for hair loss, wound healing and other degenerative skin disorders.
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