Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells

被引：0
作者：
Ruifeng Yang
Ying Zheng
Michelle Burrows
Shujing Liu
Zhi Wei
Arben Nace
Wei Guo
Suresh Kumar
George Cotsarelis
Xiaowei Xu
机构：
[1] Perelman School of Medicine,Department of Pathology and Laboratory Medicine
[2] University of Pennsylvania,Department of Dermatology
[3] Kligman Laboratories,Department of Computer Science
[4] Perelman School of Medicine,Department of Biology
[5] University of Pennsylvania,undefined
[6] New Jersey Institute of Technology,undefined
[7] University of Pennsylvania,undefined
来源：
Nature Communications | / 5卷
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摘要：
Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. The isolation and propagation of human EpSCs for tissue engineering purposes remains a challenge. Here we develop a strategy to differentiate human iPSCs (hiPSCs) into CD200+/ITGA6+ EpSCs that can reconstitute the epithelial components of the hair follicle and interfollicular epidermis. The hiPSC-derived CD200+/ITGA6+ cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. Human iPSC-derived CD200+/ITGA6+ cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. The regenerated hair follicles possess a KRT15+ stem cell population and produce hair shafts expressing hair-specific keratins. These results suggest an approach for generating large numbers of human EpSCs for tissue engineering and new treatments for hair loss, wound healing and other degenerative skin disorders.
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