Endolysin EN572-5 as an alternative to treat urinary tract infection caused by Streptococcus agalactiae

被引:1
作者
Kajsikova, Maria [1 ]
Kajsik, Michal [2 ]
Bocanova, Lucia [1 ]
Papayova, Kristina [1 ]
Drahovska, Hana [3 ]
Bukovska, Gabriela [1 ]
机构
[1] Inst Mol Biol SAS, Dept Genom & Biotechnol, Dubravska Cesta 21, Bratislava 84551, Slovakia
[2] Comenius Univ Sci Pk, Ilkovicova 8, Bratislava 84104, Slovakia
[3] Comenius Univ, Fac Nat Sci, Dept Mol Biol, Ilkovicova 6, Bratislava 84115, Slovakia
关键词
Streptococcus agalactiae; UTI GBS; Endolysin; Bacteriolytic activity; EN572-5; GROUP-B STREPTOCOCCI; ASYMPTOMATIC BACTERIURIA; BACTERIOPHAGE LYSIN; IN-VITRO; EPIDEMIOLOGY; PHAGE; SEROTYPES; DOMAIN; CPL-1; MICE;
D O I
10.1007/s00253-023-12949-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen causing urinary tract infection (UTI). Endolysin EN572-5 was identified in prophage KMB-572-E of the human isolate Streptococcus agalactiae KMB-572. The entire EN572-5 gene was cloned into an expression vector and the corresponding recombinant protein EN572-5 was expressed in Escherichia coli in a soluble form, isolated by affinity chromatography, and characterized. The isolated protein was highly active after 30 min incubation in a temperature range of - 20 degrees C to 37 degrees C and in a pH range of 5.5-8.0. The endolysin EN572-5 lytic activity was tested on different Streptococcus spp. and Lactobacillus spp. The enzyme lysed clinical GBS (n = 31/31) and different streptococci (n = 6/8), and also exhibited moderate lytic activity against UPEC (n = 4/4), but no lysis of beneficial vaginal lactobacilli (n = 4) was observed. The ability of EN572-5 to eliminate GBS during UTI was investigated using an in vitro model of UPSA. After the administration of 3 mu M EN572-5, a nearly 3-log decrease of urine bacterial burden was detected within 3 h. To date, no studies have been published on the use of endolysins against S. agalactiae during UTI. Key points center dot A lytic protein, EN572-5, from a prophage of a human GBS isolate has been identified.center dot This protein is easily produced, simple to prepare, and stable after lyophilization.center dot The bacteriolytic activity of EN572-5 was demonstrated for the first time in human urine.
引用
收藏
页码:18 / 18
页数:1
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