A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system

[1] Gao YT(2012)EASL clinical practice guidelines: management of chronic hepatitis B virus infection J Hepatol 57 167-185

[2] Han T(2010)Enhanced specificity of real-time PCR for measurement of hepatitis B virus cccDNA using restriction endonuclease and plasmid-safe ATP-dependent DNase and selective primers J Virol Method 169 181-187

[3] Li Y(2013)Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus J Clin Microbiol 51 540-546

[4] Yang B(2002)A new and sensitive method for the quantification of HBV cccDNA by real-time PCR Biochem Biophys Res Commun 295 1102-1107

[5] Wang YJ(2003)A quantitative method to detect HBV cccDNA by chimeric primer and real-time polymerase chain reaction J Virol Methods 112 45-52

[6] Wang FM(2006)Absolute and relative QPCR quantification of plasmid copy number in J Biotechnol 123 273-280

[7] Jing X(2009)Control of cccDNA function in hepatitis B virus infection J Hepatol 51 581-592

[8] Du Z(2009)Hepatitis B: the virus and disease Hepatology 49 S13-S21

[9] Hayden RT(2013)Predictability of liver-related seromarkers for the risk of hepatocellular carcinoma in chronic hepatitis B patients PLoS ONE 8 e61448-1228

[10] Gu Z(2014)Specific and nonhepatotoxic degradation of nuclear hepatitis B virus cccDNA Science 343 1221-79

[1] Gao YT(2012)EASL clinical practice guidelines: management of chronic hepatitis B virus infection J Hepatol 57 167-185

[2] Han T(2010)Enhanced specificity of real-time PCR for measurement of hepatitis B virus cccDNA using restriction endonuclease and plasmid-safe ATP-dependent DNase and selective primers J Virol Method 169 181-187

[3] Li Y(2013)Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus J Clin Microbiol 51 540-546

[4] Yang B(2002)A new and sensitive method for the quantification of HBV cccDNA by real-time PCR Biochem Biophys Res Commun 295 1102-1107

[5] Wang YJ(2003)A quantitative method to detect HBV cccDNA by chimeric primer and real-time polymerase chain reaction J Virol Methods 112 45-52

[6] Wang FM(2006)Absolute and relative QPCR quantification of plasmid copy number in J Biotechnol 123 273-280

[7] Jing X(2009)Control of cccDNA function in hepatitis B virus infection J Hepatol 51 581-592

[8] Du Z(2009)Hepatitis B: the virus and disease Hepatology 49 S13-S21

[9] Hayden RT(2013)Predictability of liver-related seromarkers for the risk of hepatocellular carcinoma in chronic hepatitis B patients PLoS ONE 8 e61448-1228

[10] Gu Z(2014)Specific and nonhepatotoxic degradation of nuclear hepatitis B virus cccDNA Science 343 1221-79