Study on breeding and fermentation characteristics of Saccharomyces cerevisiae for Malus asiatica wine

Saccharomyces cerevisiae D65 isolated from the Malus asiatica naturally fermented liquid was used as the parental strain for mutation breeding of atmospheric and room temperature plasma (ARTP). The mutant strains with excellent characteristics were obtained through acid resistance test, esterase activity test, and fermentation test multi-step screening. The results showed that the specific activity of total esterase of D65-4D10 was 2428.30 U·g−1, and the ester content in the fermented Malus asiatica wine was 5710.75 ug·L−1, which was 20.69% and 41.64% higher than that of the parental strain, respectively. Moreover, the Malus asiatica wine fermented by D65-4D10 had stronger antioxidant capacity than the control, the highest score in sensory evaluation, and completed fermentation 24 h earlier than commercial yeast. After seven generations of continuous culture, the fermentation performance of D65-4D10 remained stable and had the potential to be applied to Malus asiatica wine fermentation.