Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing

被引：0
作者：
Chad Nusbaum
Toshiro K Ohsumi
James Gomez
John Aquadro
Thomas C Victor
Robert M Warren
Deborah T Hung
Bruce W Birren
Eric S Lander
David B Jaffe
机构：
[1] Broad Institute of Massachusetts Institute of Technology, Harvard University, Cambridge, MA 02141
[2] Department of Science and Technology, National Research Foundation Centre of Excellence in Biomedical Tuberculosis Research, Stellenbosch University, Tygerberg 7505 Cape Town
[3] Department of Molecular Biology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
[4] Whitehead Institute for Biomedical Research, Cambridge, MA 02139
[5] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139
[6] Department of Systems Biology, Harvard Medical School, Boston, MA 02115
[7] Department of Molecular Biology, Massachusetts General Hospital, Richard B. Simches Research Center, Boston, MA 02114
来源：
Nature Methods | 2009年 / 6卷 / 1期
关键词：
D O I：
10.1038/nmeth.1286
中图分类号：
学科分类号：
摘要：
Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected ∼98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.
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页码：67 / 69
页数：2
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