16S rDNA clone library-based bacterial phylogenetic diversity associated with three South China Sea sponges

被引：0
作者：
Zhiyong Li
Ye Hu
Yan Liu
Yi Huang
Liming He
Xiaoling Miao
机构：
[1] Shanghai Jiao Tong University,Marine Biotechnology Laboratory, College of Life Science and Biotechnology
[2] Shanghai Jiao Tong University,Key Laboratory of Microbial Metabolism, Ministry of Education, China
来源：
World Journal of Microbiology and Biotechnology | 2007年 / 23卷
关键词：
Sponge; Bacterial diversity; 16S rDNA clone library; Restriction fragment length polymorphism; Phylogenetic analysis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Culture-independent molecular techniques, 16S rDNA clone library alongside RFLP and phylogenetic analysis, were applied to investigate the bacterial diversity associated with three South China Sea sponges, Stelletta tenui, Halichondria rugosa and Dysidea avara. A wide bacterial diversity was detected according to total genomic DNA-based 16S rDNA clone library, abundant clones with low identify with sequences retrieved from database were found as well as uncultured sponge symbionts. The phylogenetic analysis shows that the bacterial community structure of Stelletta tenui is similar to that of Halichondria rugosa comprising gamma-Proteobacteria and Firmicutes. Whereas, alpha-Proteobacteria, gamma-Protebacteria, Bacteroidetes and uncultured sponge symbionts were found in sponge Dysidea avara, suggesting that Dysidea avara has the highest bacteria diversity among these sponges. A specific sponge–microbe association is suggested based on the difference of bacterial diversity among these three sponges from the same geography location and the observed sponge species-specific bacteria.
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页码：1265 / 1272
页数：7
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