Optimization of culture media for Bacillus species by statistical experimental design methods

被引：0
作者：
Jae-Hee Cho
Young-Bum Kim
Eun-Ki Kim
机构：
[1] Inha University,Department of Biological Engineering
来源：
Korean Journal of Chemical Engineering | 2009年 / 26卷
关键词：
Optimization of Media; Species; Plackett-Burman Design; Box-Behnken Design; Response Surface Method;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The medium compositions of Bacillus species (three strains) were optimized sequentially through two statistical experimental designs, Plackett-Burman and the Box-Behnken, which were determined by representative three-factor response-surface methodology. pH and DO control was found to enhance the viable cell number of Bacilli. Most strains grew well in the optimum condition and reached nearly up to of 5×109 CFU/mL. Additionally, when three of the Bacilli were cultivated in the optimized medium with pH and DO control, the viable cell number increased 3.6 times in B. subtilis, 64.1 times in B. licheniformis and 8.3 times in B. coagulans, respectively, in comparison with the original industrial medium.
引用
收藏
页码：754 / 759
页数：5
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