Possible attenuation of the G2 DNA damage cell cycle checkpoint in HeLa cells by extremely low frequency (ELF) electromagnetic fields

被引：23

作者：

Harris P.A. ^{[1
]}

Lamb J. ^{[2
]}

Heaton B. ^{[3
]}

Wheatley D.N. ^{[4
]}

机构：

[1] Section of Surgery, Division of Clinical Sciences, University of Sheffield, Sheffield S5 7AU, Herries Road

[2] Department of Adult Oncology, Dana Farber Cancer Institute, Boston, MA 02115

[3] Dept. of BioMedical Phys./BioEng., University of Medical Buildings, Aberdeen AB25 2ZD, Foresterhill

[4] Department of Cell Pathology, University of Aberdeen, Aberdeen AB24 5UA

来源：

Cancer Cell International | / 2卷 / 1期

关键词：

Magnetic Field Exposure; Double Thymidine Block; Murine L929 Fibroblast; Helmholtz Pair; Mild Thermal Stress;

D O I：

10.1186/1475-2867-2-3

中图分类号：

学科分类号：

摘要：

Background: The issue remains unresolved as to whether low frequency magnetic fields can affect cell behaviour, with the possibility that they may be in part responsible for the increased incidence of leukaemia in parts of the population exposed to them. Methods: Combined treatment of HeLa cells with gamma-irradiation (1, 3 and 5 Grays) and extra low frequency magnetic fields of ∼50 Hz was carried out under rigorously controlled conditions. Results: Synchronised cells progressing from S-phase arrived at mitosis on average marginally ahead of irradiation controls not exposed to ELF. In no instance out of a total of twenty separate experiments did this "double-insult" further delay entry of cells into mitosis, as had been anticipated. Conclusion: This apparently "non-genotoxic" agent (ELF) appears to be capable of affecting cells that would normally arrest for longer in G2, suggesting a weakening of the stringency of the late cycle (G2) checkpoint. © 2002 Harris et al; licensee BioMed Central Ltd.

引用

页数：11

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