Photodynamic inactivation of Klebsiella pneumoniae biofilms and planktonic cells by 5-aminolevulinic acid and 5-aminolevulinic acid methyl ester

被引：0
作者：
Chengcheng Liu
Yingli Zhou
Li Wang
Lei Han
Jin’e Lei
Hafiz Muhammad Ishaq
Sean P. Nair
Jiru Xu
机构：
[1] School of Basic Medical Sciences,Department of Pathogenic Microbiology and Immunology
[2] Xi’an Jiaotong University Health Science Center,The First Affiliated Hospital of College of Medicine
[3] Xi’an Jiaotong University,Department of Microbial Diseases
[4] UCL Eastman Dental Institute,undefined
来源：
Lasers in Medical Science | 2016年 / 31卷
关键词：
PACT; Biofilms; 5-ALA; MAL;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The treatment of Klebsiella pneumoniae, particularly extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae, is currently a great challenge. Photodynamic antimicrobial chemotherapy is a promising approach for killing antibiotic-resistant bacteria. The aim of this study was to evaluate the capacity of 5-aminolevulinic acid (5-ALA) and its derivative 5-ALA methyl ester (MAL) in the presence of white light to cause photodynamic inactivation (PDI) of K. pneumoniae planktonic and biofilm cells. In the presence of white light, 5-ALA and MAL inactivated planktonic cells in a concentration-dependent manner. Biofilms were also sensitive to 5-ALA and MAL-mediated PDI. The mechanisms by which 5-ALA and MAL caused PDI of ESBL-producing K. pneumonia were also investigated. Exposure of K. pneumonia to light in the presence of either 5-ALA or MAL induced cleavage of genomic DNA and the rapid release of intracellular biopolymers. Intensely denatured cytoplasmic contents and aggregated ribosomes were also detected by transmission electron microscopy. Scanning electron microscopy showed that PDI of biofilms caused aggregated bacteria to detach and that the bacterial cell envelope was damaged. This study provides insights into 5-ALA and MAL-mediated PDI of ESBL-producing K. pneumoniae.
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页码：557 / 565
页数：8
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共 53 条
  • [11] Mroz P(2010)Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms Laser Med Sci 32 1292-1327
  • [12] Hamblin MR(2012)Factors influencing the susceptibility of gram-negative bacteria to toluidine blue O-mediated lethal photosensitization Med Res Rev 184 1013-1021
  • [13] Wood S(2013)Effective photodynamic therapy against microbial populations in human deep tissue abscess aspirates J Surg Res 52 1366-1373
  • [14] Komerik N(2008)Comparison of the efficacy of rose bengal and erythrosine in photodynamic therapy against Animicrob Agents Chemother 3 430-435
  • [15] Wilson M(2004)Photodynamic therapy based on 5-aminolevulinic acid and its use as an antimicrobial agent Photochem Photobiol Sci 17 S24-28
  • [16] Haidaris CG(2011)Effects of 5-aminolevulinic acid-mediated photodynamic therapy on antibiotic-resistant staphylococcal biofilm: an Hong Kong Med J 55 860-866
  • [17] Rossoni RD(2011) study Antimicrob Agents Chemother 23 3359-3368
  • [18] Harris F(2002)Effects on gram-negative and gram-positive bacteria mediated by 5-aminolevulinic acid and 5-aminolevulinic acid derivatives Biomaterials 1760 104-109
  • [19] Pierpoint L(2006)ALA induced photodynamic effects on gram positive and negative bacteria Biochim Biophys Acta 6 52-61
  • [20] Li X(2009)Photodynamic inactivation of multi-drug resistant pathogens in Hong Kong Photodiagn Photodyn Ther 125 179-187
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