Protein Scission by Metal Ion–Ascorbate System

被引:0
|
作者
Jolanta Sereikaite
Jelena Jachno
Rasa Santockyte
Piotr Chmielevski
Vladas-Algirdas Bumelis
Gervydas Dienys
机构
[1] Vilnius Gediminas Technical University,Department of Chemistry and Bioengineering
[2] Institute of Biotechnology,undefined
来源
The Protein Journal | 2006年 / 25卷
关键词
Ascorbate; human growth hormone; metal binding site; metal ions; protein scission;
D O I
暂无
中图分类号
学科分类号
摘要
About 14 proteins were tested for specific oxidative scission catalyzed by metal ions in the presence of ascorbate and oxidizing agents (O2 or hydrogen peroxide). Only four of them were degraded by Fe3+/Fe2+- ascorbate, twelve – by Cu2+/Cu+-ascorbate and two proteins (α- and β-caseins) were degraded by Pd2+ ions. The rate and the intensity of degradation are very different for various proteins. For the most of tested proteins only a small fraction of molecules was degraded. None of them was degraded completely. Two possible reasons of protein stability against oxidative degradation may be proposed as follows: either there is no metal binding site in a protein molecule, or metal binding ligands of protein undergo a rapid oxidative modification and the metal ion is released from the binding site. Human growth hormone was cut specifically at two sites by Cu2+/Cu+-ascorbate system. At least one of amino acid residues of this protein was modified by formation of reactive carbonyl.
引用
收藏
页码:369 / 378
页数:9
相关论文
共 50 条
  • [41] Ascorbate does not act as a pro-oxidant towards lipids and proteins in human plasma exposed to redox-active transition metal ions and hydrogen peroxide
    Suh, J
    Zhu, BZ
    Frei, B
    FREE RADICAL BIOLOGY AND MEDICINE, 2003, 34 (10) : 1306 - 1314
  • [42] Predicting Wear and Blood Metal Ion Levels in Metal-on-Metal Hip Resurfacing
    Matthies, Ashley K.
    Henckel, Johann
    Cro, Suzie
    Suarez, Alexander
    Noble, Philip C.
    Skinner, John
    Hart, Alister J.
    JOURNAL OF ORTHOPAEDIC RESEARCH, 2014, 32 (01) : 167 - 174
  • [43] What Happens to Serum Metal Ion Levels After a Metal-on-Metal Bearing is Removed?
    Ball, Scott T.
    Severns, Dustyn
    Linn, Michael
    Meyer, R. Scott
    Swenson, F. Craig
    JOURNAL OF ARTHROPLASTY, 2013, 28 (08): : 53 - 55
  • [44] The association of metal ion exposure with α-synuclein-like immunoreactivity in the central nervous system of fish, Catostomus commersoni
    Boudreau, Heather S.
    Krol, Karmen M.
    Eibl, Joseph K.
    Williams, Linda D.
    Rossiter, John P.
    Palace, Vincent P.
    Ross, Gregory M.
    AQUATIC TOXICOLOGY, 2009, 92 (04) : 258 - 263
  • [45] Role of vitamin E in ascorbate-dependent protein thiol oxidation in rat liver endoplasmic reticulum
    Csala, M
    Szarka, A
    Margittai, É
    Mile, V
    Kardon, T
    Braun, L
    Mandl, J
    Bánhegyi, G
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 388 (01) : 55 - 59
  • [46] In vitro evidence for UV-protection of the eye by the corneal epithelium mediated by the cytoplasmic protein, RNA, and ascorbate
    Ringvold, A
    ACTA OPHTHALMOLOGICA SCANDINAVICA, 1997, 75 (05): : 496 - 498
  • [47] The NRAMP family of metal-ion transporters
    Nevo, Yaniv
    Nelson, Nathan
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2006, 1763 (07): : 609 - 620
  • [48] Luminescent Silicon Nanocrystals as Metal Ion Sensors
    Stacy, Benjamin J.
    Nagasaki, Kara
    Korgel, Brian A.
    ACS NANO, 2024, 18 (24) : 15744 - 15753
  • [49] Development of Metal Ions Analysis by Ion Chromatography
    Yu Hong
    Wang Yuxin
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2007, 25 (03) : 303 - 309
  • [50] METAL ION SORPTION TO BIRCH AND SPRUCE WOOD
    Su, Pingping
    Granholm, Kim
    Pranovich, Andrey
    Harju, Leo
    Holmbom, Bjarne
    Ivaska, Ari
    BIORESOURCES, 2012, 7 (02): : 2141 - 2155