Molecular Cloning and Characterization of a Novel Human C4orf13 Gene, Tentatively a Member of the Sodium Bile Acid Cotransporter Family

被引：0
作者：
Xianqiong Zou
Dianzuo Wang
Guanzhou Qiu
Chaoneng Ji
Feng Jin
Maoqing Wu
Huarui Zheng
Xin Li
Liyun Sun
Yu Wang
Rong Tang
Robert Chunhua Zhao
Yumin Mao
机构：
[1] Central South University,School of Resource Processing and Bio
[2] Fudan University,engineering
[3] Chinese Academy of Medical Science and Peking Union Medical College,State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science
来源：
Biochemical Genetics | 2005年 / 43卷
关键词：
sodium bile acid cotransporter family; human C4orf13; expression; RT-PCR;
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摘要：
By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA (C4orf13). This cDNA is 2706 bp in length, encoding a 340-amino-acid polypeptide that contains a typical SBF (sodium bile acid cotransporter family) domain and ten possible transmembrane segments. The putative protein C4orf13 shows high similarity with its orthologs in Mus musculus and Xenopus laevis. Human C4orf13 is mapped to chromosome 4q31.2 and contains 12 exons. RT-PCR analysis shows that human C4orf13 is widely expressed in human tissues, and the expression levels in liver and lung are relatively high, expression levels in placenta, kidney, spleen, and thymus are moderate, low levels of expression are detected in heart, prostate, and testis.
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页码：165 / 173
页数：8
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  • [1] Alcalay M.(1988)CpG islands of the X chromosome are gene associated. Nucleic acids Res. 16 9527-9543
  • [2] Toniolo D.(1997)Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Yeast 13 819-828
  • [3] Bobrowicz P.(1998). Am. J. Physiol. 274 G157-G169
  • [4] Wysocki R.(1991)Expression and transport properties of the human ileal and renal sodium-dependent bile acid transporter. Proc. Natl. Acad. Sci. U.S.A. 88 10629-10633
  • [5] Owsianik G.(1992)Functional expression cloning and characterization of the hepatocyte Na+/bile acid cotransport system. Biochemistry 31 4737-4749
  • [6] Goffeau A.(2000)Bile acids biosynthesis, Genomics 69 203-213
  • [7] Ulaszewski S.(1964)Structural and functional characterization of liver cell-specific activity of the human sodium/taurocholate cotransporter. Am. J. physiol. 207 964-970
  • [8] Craddock A. L.(1981)Renal excretion of bile acids: taurocholate, glycocholate, and cholic acids. J. Clin. Invest. 67 1141-1150
  • [9] Love M. W.(1995)Sodium-coupled taurocholate transport in the proximal convolution of the rat kidney in vivo and in vitro. J. Biol. Chem. 270 27228-27234
  • [10] Daniel R. W.(undefined)Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity, undefined undefined undefined-undefined
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