Age and Visual Experience-dependent Expression of NMDAR1 Splice Variants in Rat Retina

被引：0
作者：
Georgia Manta
Athanasios D. Spathis
Stavros Taraviras
Elias D. Kouvelas
Adamantia Mitsacos
机构：
[1] University of Patras,Department of Physiology, School of Medicine
[2] Academy of Athens,Biomedical Research Foundation
来源：
Neurochemical Research | 2011年 / 36卷
关键词：
NMDA; NMDAR1; Retina; Dark rearing; Alternative splicing;
D O I：
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中图分类号：
学科分类号：
摘要：
The N-methyl-D-aspartate receptor (NMDAR) is a key molecule mediating brain plasticity related processes. Knowing that alternative splicing of the NMDAR1 (NR1) subunit offers molecular diversity to NMDAR, controls the forward trafficking of the NR1 protein and is important for placing NMDA receptors at synapses, we investigated herein the postnatal developmental expression and the influence of visual deprivation on NR1 subunit splice variants in rat retina. Real-time PCR was performed using oligonucleotide primers specific for N- terminal (NR1a, NR1b) and C-terminal splice variants (NR1-1, NR1-2, NR1-3, NR1-4). The developmental profiles of mRNA expression levels of all NR1 isoforms peaked at the end of the third week. Dark rearing led to reductions in both N- and C-terminal NR1 variants in several developmental ages and a significant interaction between age and visual experience was observed for NR1a, NR1-2 and NR1-4 expression. Our results have demonstrated a developmental and visual experience-dependent regulation of NR1 splicing in rat retina.
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页码：1417 / 1425
页数：8
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