Bioaerosol emission from wastewater treatment plants

被引：82

作者：

Leonor Pascual

Sara Pérez-Luz

M. Adela Yáñez

Ana Santamaría

Karina Gibert

Miquel Salgot

David Apraiz

Vicente Catalán

机构：

[1] Labaqua S.A., Alicante

[2] Depto. Estadistica Invest. Operativa, Fac. de Matematicas y Estadistica, Universidad Politecnica de Cataluna, Barcelona

[3] Departamento de Productos Naturales, Facultad de Farmacia, Universidad de Barcelona, Barcelona

[4] LABAQUA C/ Alona 33

来源：

Aerobiologia | 2003年 / 19卷 / 3-4期

关键词：

Bioaerosols; Fecal coliforms; Heterotrophic plate count; Moulds and yeasts; Mycobacterium tuberculosis; PCR; Pseudomonas aeruginosa; Total coliforms; Wastewater treatment plants;

D O I：

10.1023/B:AERO.0000006598.45757.7f

中图分类号：

学科分类号：

摘要：

In this report we describe the results of a study conducted to better estimate airborne microorganisms present in wastewater treatment plants (WWTP) environments. Air samples were taken with the Air Sampler MAS 100 impactor (Merck) at each stage of an activated-sludge WWTP (pre-treatment, primary clarifiers, aeration basins and sludge processors), in order to determine levels of contamination according to work stations. Culture methods were used to investigate heterotrophic plate count, moulds and yeasts, total and fecal coliforms, Pseudomonas aeruginosa and species belonging to Mycobacterium tuberculosis complex, whereas these last microorganisms were also analysed by Polymerase Chain Reaction (PCR) methods. Statistical analysis showed that pretreatment and primary clarifiers were the stages with the highest emission of bioaerosols, and that bioaerosol concentration at each stage depended on wind speed and daily inflow at the WWTP. Low concentrations of P. aeruginosa were found isolated at pretreatment and primary clarifiers; however, species of M. tuberculosis complex were not detected at any stage of the treatment plant.

引用

页码：261 / 270

页数：9

共 33 条

[1]

Alvarez A.J., Buttner M.P., Toranzos G.A., Dvorsky E.A., Toro A., Heikes T.B., Mertikas-Pifer L.E., Stetzenbach L.D., Use of solid-phase PCR for enhanced detection of airborne microorganisms, Appl. Environ. Microbiol., 60, pp. 374-376, (1994)

[2]

Alvarez A.J., Buttner M.P., Stetzenbach L.D., PCR for bioaerosol monitoring: Sensitivity and environmental interference, Appl. Environ. Microbiol., 61, pp. 3639-3644, (1995)

[3]

Boutin P., Moline J., Torre M., Boissinot E., Contamination atmosphérique et respiratoire par les aérosols d'Eaux résiduares, Rev. Mal. Resp., 1, pp. 125-131, (1984)

[4]

Boutin P., Torre M., Moline J., Boissinot E., Bacterial atmospheric contamination in wastewater treatment plants, Advances in Aerobiology, 51, pp. 365-370, (1987)

[5]

Buttner M.P., Stetzenbach L.D., Evaluation of four aerobiological sampling methods for the retrieval of aerosolized Pseudomonas syringae, Appl. Environ. Microbiol., 57, pp. 1268-1270, (1991)

[6]

Catalan V., Garcia F., Moreno C., Apraiz D., Detection of Legionella pneumophila in wastewater by nested polymerase chain reaction, Res. Microbiol., 148, pp. 71-78, (1997)

[7]

Contamination bacterienne de l'Atmosphère par les stations de traitement d'Eaux résiduares, Informations Techniques, 65, pp. 1-3, (1987)

[8]

Feller W., An Introduction to the Probability Theory and Its Application, (1950)

[9]

Flescher A.R., Kasper D.L., Modern P.A., Mason Jr. E.O., Legionella pneumophila growth inhibition by human pharyngeal Flora, J. Infect. Dis., 142, pp. 313-317, (1980)

[10]

Heidelberg J.F., Shahamat M., Levin M., Rahman I., Stelma G., Grim C., Colwell R.R., Effect of aerosolization on culturability and viability of gram-negative bacteria, Appl. Environ. Microbiol., 63, pp. 3585-3588, (1997)

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