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Epithelial Na+ channels and stomatin are expressed in rat trigeminal mechanosensory neurons
被引：0
作者：
B. Fricke
R. Lints
G. Stewart
H. Drummond
G. Dodt
M. Driscoll
M. von Düring
机构：
[1] Department of Neuroanatomy,
[2] Institute of Anatomy MA6/152,undefined
[3] Ruhr University,undefined
[4] Universitätsstrasse 150,undefined
[5] D-44801 Bochum,undefined
[6] Germany,undefined
[7] Department of Molecular Biology and Biochemistry,undefined
[8] Rutgers,undefined
[9] The State University of New Jersey,undefined
[10] 604 Allison Road,undefined
[11] Piscataway,undefined
[12] NJ 08854,undefined
[13] USA,undefined
[14] Department of Medicine,undefined
[15] University College London School of Medicine,undefined
[16] Rayne Institute,undefined
[17] 5 University Street,undefined
[18] London,undefined
[19] UK,undefined
[20] Department of Internal Medicine,undefined
[21] Howard Hughes Medical Institute,undefined
[22] University of Iowa College of Medicine,undefined
[23] Iowa City,undefined
[24] IA 52242,undefined
[25] USA,undefined
[26] Institute of Physiological Chemistry,undefined
[27] Ruhr University,undefined
[28] Universitätsstrasse 150,undefined
[29] D-44801 Bochum,undefined
[30] Germany,undefined
关键词：
Epithelial sodium channel Stomatin Mechanotransduction Trigeminal somatosensory nervous system Vibrissae Ion channels Immunocytochemistry Rat (Wistar);
D O I：
10.1007/s004419900153
中图分类号：
学科分类号：
摘要：
Caenorhabditis elegans MEC-4 and MEC-10 are subunits of the degenerin/epithelial Na+ channel (DEG/ENaC) ion channel superfamily thought to be associated with MEC-2 (a stomatin-like protein) in a mechanotransducing molecular complex in specialized touch sensory neurons. A key question is whether analogous molecular complexes in higher organisms transduce mechanical signals. To address this question, we selected mechanoreceptors of the rat vibrissal follicle-sinus complex in the mystacial pad and the trigeminal ganglia for an immunocytochemical and molecular biological study. RT-PCR of poly(A+) mRNA of rat trigeminal ganglia indicated that α-, β-, and γ-ENaC and stomatin mRNA are expressed in rat trigeminal ganglia. Using immunocytochemistry, we found that α-, β-, and γ-ENaC subunits and stomatin are localized in the perikarya of the trigeminal neurons and in a minor fraction of their termination site in the vibrissal follicle-sinus complex, where longitudinal lanceolate endings are immunopositive. We conclude that α-, β-, and γ-ENaC subunits as well as the candidate interacting protein stomatin are coexpressed in a mammalian mechanoreceptor, a location consistent with a possible role in mechanotransduction.
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页码：327 / 334
页数：7
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