Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan

被引：14

作者：

Misawa K. ^{[1
]}

Tarumoto N. ^{[2
,3
]}

Tamura S. ^{[4
]}

Osa M. ^{[1
]}

Hamamoto T. ^{[5
]}

Yuki A. ^{[5
]}

Kouzaki Y. ^{[1
]}

Imai K. ^{[1
]}

Ronald R.L. ^{[6
]}

Yamaguchi T. ^{[2
,3
]}

Murakami T. ^{[3
,7
]}

Maesaki S. ^{[2
,3
]}

Suzuki Y. ^{[6
]}

Kawana A. ^{[1
]}

Maeda T. ^{[5
,7
]}

机构：

[1] Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Saitama

[2] Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama

[3] Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama

[4] Department of Pediatrics, National Defense Medical College, Saitama

[5] Department of Laboratory Medicine, National Defense Medical College Hospital, Saitama

[6] Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, University of Tokyo, Chiba

[7] Department of Microbiology, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Haemophilus influenzae; Penicillin binding protein; SNP; β-Lactamase-negative ampicillin-resistant (BLNAR);

D O I：

10.1186/s13104-018-3169-0

中图分类号：

学科分类号：

摘要：

Objective: β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this study were to perform comprehensive genetic analyses of whole regions of the penicillin-binding proteins in H. influenzae and to identify additional single nucleotide polymorphisms related to antibiotic resistance, especially to ampicillin and other cephalosporins. Results: In this genome analysis of the ftsI gene in 27 strains of H. influenzae, 10 of 23 (43.5%) specimens of group III genotype β-lactamase-negative ampicillin-resistant H. influenzae were paradoxically classified as ampicillin-sensitive phenotypes. Unfortunately, we could not identify any novel mutations that were significantly associated with ampicillin minimum inhibitory concentrations in other regions of the penicillin-binding proteins, and we reconfirmed that susceptibility to β-lactam antibiotics was mainly defined by previously reported SNPs in the ftsI gene. We should also consider detailed changes in expression that lead to antibiotic resistance in the future because the acquisition of resistance to antimicrobials can be predicted by the expression levels of a small number of genes. © 2018 The Author(s).

引用

共 32 条

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