GAPDH spike RNA as an alternative for housekeeping genes in relative gene expression assay using real-time PCR

被引:0
作者
Zeenah Weheed Atwan
机构
[1] University of Basrah,Biology Department, College of Science
关键词
Spike RNA; qPCR; Relative expression; Housekeeping genes; Universal normalizer;
D O I
10.1186/s42269-020-00284-1
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 112 条
  • [1] Ayers D(2007)Expression stability of commonly used reference genes in canine articular connective tissues BMC Vet Res 3 7-395
  • [2] Clements DN(2005)GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues Physiol Genomics 21 389-193
  • [3] Salway F(2000)Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays J Mol Endocrinol 25 169-265
  • [4] Day PJR(2016)Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis Braz J Microbiol 47 259-417
  • [5] Barber RD(2009)Validation of endogenous internal real-time PCR controls in renal tissues Am J Nephrol 30 413-119
  • [6] Harmer DW(2004)Validation of housekeeping genes for normalizing RNA expression in real-time PCR Biotechniques. 37 112-1001
  • [7] Coleman RA(1996)A novel method for real time quantitative RT-PCR Genome Res 6 995-627
  • [8] Clark BJ(2001)Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes Horm Metab Res 33 625-206
  • [9] Bustin SA(1993)Kinetic PCR analysis: real-time monitoring of DNA amplification reactions Nat Biotechnol 11 1026-306
  • [10] Chen C(2005)Real-time RT-PCR normalisation; strategies and considerations Genes Immun 6 279-722