Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

被引：92

作者：

Mohapatra S. ^{[1
]}

Mohanta P.R. ^{[1
]}

Sarkar B. ^{[2
]}

Daware A. ^{[2
]}

Kumar C. ^{[3
]}

Samantaray D.P. ^{[1
]}

机构：

[1] Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Odisha

[2] National Institute of Abiotic Stress Management, Malegaon, Baramati, Pune, 413115, Maharashtra

[3] Department of Chemistry, Sheila Dhar Institute of Soil Science, University of Allahabad, Allahabad, 211002, Uttar Pradesh

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2017年 / 87卷 / 2期

关键词：

Bacillus; Bio-plastic; NMR; Polyhydroxyalkanoates; Waste water;

D O I：

10.1007/s40011-015-0626-6

中图分类号：

学科分类号：

摘要：

Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view of that, different Bacillus species were isolated from municipal sewage and screened for evaluating their efficacy of PHA production. Growth parameters such as temperature, pH, glucose concentration and carbon nitrogen combinations were optimized with respect to higher biomass production as it is analogous to PHA accumulation. Under optimized conditions, the Bacillus species produced 3.09 g/L of PHAs which was estimated as a higher yield in comparison to other similar strains. Fourier transform infrared spectroscopic analysis of the extracted polyhydroxybutyrate confirmed the distinct peak corresponding to C=O group, whereas proton nuclear magnetic resonance (1H NMR) and differential scanning colorimetric analysis exhibited detailed insight of its chemical structure and properties by reflecting monomeric unit. The high yielding bacterial isolate was identified by 16S rDNA sequencing and the sequence was confirmed as Bacillus subtilis with an accession no. KP172548 after submission to NCBI data base. The potential bacterium may be further exploited for cost effective and mass scale production of biopolymer. © 2015, The National Academy of Sciences, India.

引用

页码：459 / 466

页数：7

共 41 条

[1]

Chanprateep S., Current trends in biodegradable polyhydroxyalkanoates, J Biosci Bioeng, 110, 6, pp. 621-632, (2010)

[2]

Mani D., Kumar C., Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation, Int J Environ Sci Technol, 11, 3, pp. 843-872, (2014)

[3]

Braunegg G., Lefebvre G., Genser K.F., Polyhydroxyalkanoates, biopolyesters from renewable resources: physiological and engineering aspects, J of Biotechnol, 65, pp. 127-161, (1998)

[4]

Koller M., Gasser I., Schmid F., Berg G., Linking ecology with economy: insights into polyhydroxyalkanoate-producing microorganisms, Eng Life Sci, 11, 3, pp. 222-237, (2011)

[5]

Bhuwal A.K., Singh G., Aggarwal N.K., Goyal V., Yadav A., Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industry wastes, J Biomater, (2013)

[6]

Khanna S., Srivastava A.K., Recent advances in microbial polyhydroxyalkanoates, Proc Biochem, 40, 2, pp. 607-619, (2005)

[7]

Kulpreecha S., Boonruangthavorn A., Meksiriporn B., Thongchul N., Inexpensive fed-batch cultivation for high poly (3-hydroxybutyrate) production by a new isolate of Bacillus megaterium, J Biosci Bioeng, 107, 3, pp. 240-245, (2009)

[8]

Pandian S.R.K., Deepak V., Kalishwaralal K., Rameshkumar N., Jeyaraj M., Gurunathan S., Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3, Bioresource Technol, 101, pp. 705-711, (2010)

[9]

Biedendieck R., Gamer M., Jaensch L., Meyer S., Rohde M., Deckwer W.D., Jahn D.A., Sucrose inducible promoter system for the intra- and extracellular protein production in B. megaterium, J Biotechnol, 132, pp. 426-430, (2007)

[10]

Khiyami M.A., Al Fadual S.M., Bahklia A.H., Polyhydroxyalkanoates production via Bacillus plastic composite support (PCS) biofilm and date palm syrup, J Med Plants Res, 5, 14, pp. 3312-3320, (2011)

← 1 2 3 4 5 →