Callus induction and plant regeneration in Alternanthera philoxeroides

被引:0
作者
Jianming Gao
Jinzhi Li
Caibo Luo
Liang Yin
Shengchun Li
Guangxiao Yang
Guangyuan He
机构
[1] Huazhong University of Science & Technology (HUST),China
[2] Chinese Academy of Tropical Agricultural Sciences (CATAS),UK HUST
[3] Taizhou University,RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International Cooperation base of Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Sc
来源
Molecular Biology Reports | 2011年 / 38卷
关键词
(Mart.) Griseb; Callus induction; Orthogonal design; Plantlet regeneration;
D O I
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中图分类号
学科分类号
摘要
To establish a successful in vitro plant regeneration system in Alternanthera philoxeroides (Mart.) Griseb, an orthogonal design was used to investigate the effects of three factors (plant growth regulators, explant types and dark treatment in initial-stage), each having three levels. The effects of these factors and levels on callus induction and shoot regeneration were quantitatively evaluated by analysis of variance. The experimental results showed that the callus induction was significantly affected by 2, 4-dichlorophenoxyacetic acid (2, 4-D), and shoot differentiation from subcultured pieces of callus was enhanced mostly by dark treatment in initial-stage. The optimal conditions for callus induction are obtained from the stem explants cultured on semi-solid Murashige and Skoog (MS) medium plus 2.2 μM BA and 2.2 μM 2, 4-D, with 20 days dark treatment in initial-stage. The highest frequency of shoot regeneration is obtained from the calli cultured on semi-solid MS medium plus 8.8 μM BA, without dark treatment in initial-stage.
引用
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页码:1413 / 1417
页数:4
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