Augmentation of anti-Fas antibody-mediated apoptosis on human glioma cells by liposomes associated with the antibody

被引：0
作者：
Seiji Ohta
Masaaki Mizuno
Toru Takaoka
Jun Yoshida
机构：
[1] Nagoya University School of Medicine,Department of Neurosurgery
[2] National Institute for Longevity Sciences,undefined
来源：
Journal of Neuro-Oncology | 1997年 / 35卷
关键词：
apoptosis; glioma; anti-Fas antibody; liposome;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We investigated the susceptibility of five human glioma cell lines toanti-Fas antibody. All human glioma cells tested constitutively expressedFas antigen on their surfaces and the level of the expression variedslightly in each cell line. The cells had a low susceptibility to anti-Fasantibody-mediated apoptosis. There were four moderately resistant cell lines(U251-SP, U251-MG, SK-MG-1, T98) and one highly resistant cell line (U251nu/nu). For this study we prepared liposomes containing anti-Fas antibodyand studied the augmentation of the antibody-mediated apoptosis. Theliposomes induced apoptosis significantly more often than did anti-Fasantibody alone. These results indicate that anti-Fas antibody-mediatedapoptosis does not require a critical level of cell surface expression ofFas antigen but rather depends on the intensity of Fas signal transduction.
引用
收藏
页码：7 / 11
页数：4
相关论文
共 61 条
[1]  
Yoshida J(1994)Long-term follow-up results of 175 patients with malignant glioma: Importance of radical tumour resection and postoperative adjuvant therapy with interferon, ACNU and radiation Acta Neurochir (Wien) 127 55-59
[2]  
Kajita Y(1989)A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor J Exp Med 169 1747-1756
[3]  
Wakabayashi T(1994)Divergent signaling via APO-1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death EMBO J 13 4587-4596
[4]  
Sugita K(1993)Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family Cell 75 1169-1178
[5]  
Yonehara S(1994)Anti-Fas antibody-mediated apoptosis of cultured human glioma cells. Induction and modulation of sensitivity by cytokines J Clin Invest 94 954-964
[6]  
Ishii A(1995)Fas gene transfer for human malignant glioma Cancer Res 55 2936-2944
[7]  
Yonehara M(1993)Efficient gene transfer with less than cytotoxicity by means of cationic multilamellar liposomes Biochem Biophys Res Commun 196 1042-1048
[8]  
Schulze-Osthoff K(1995)Simple preparation and characterization of cationic liposomes associated with a monoclonal antibody against glioma-associated antigen (immunoliposomes) J Liposome Res 5 981-995
[9]  
Krammer PH(1994)Retroviral transfer of herpes simplex thymidine kinase gene into glioma cells caused targeting of gancyclovir cytotoxic effect Neurol Med Chir 34 339-344
[10]  
Droge W(1990)Growth inhibition of glioma cells transfected with the human ?-interferon gene by liposomes coupled with a monoclonal antibody Cancer Res 50 7826-7829
1234567