Antioxidant effects of oleuropein versus oxidative stress induced by ethanol in the rat intestine

被引：13

作者：

Alirezaei M. ^{[1
]}

Dezfoulian O. ^{[2
]}

Sookhtehzari A. ^{[3
]}

Asadian P. ^{[3
]}

Khoshdel Z. ^{[4
]}

机构：

[1] Division of Biochemistry, School of Veterinary Medicine, Lorestan University, P. O. Box: 465, Khorramabad

[2] Department of Pathobiology, School of Veterinary Medicine, Lorestan University, P. O. Box: 465, Khorramabad

[3] Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, P. O. Box: 465, Khorramabad

[4] Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad

来源：

Comparative Clinical Pathology | 2014年 / 23卷 / 5期

关键词：

CAT; Ethanol; GPx; Intestine; Oleuropein; Rat;

D O I：

10.1007/s00580-013-1791-8

中图分类号：

学科分类号：

摘要：

Purified oleuropein from olive leaf extract indicated antioxidant properties in our previous reports. The recent study demonstrated that the lesions of absolute ethanol could be attributed to the increasing reactive oxygen species, subsequently lipid peroxidation and gastric ulcers. Therefore, the antioxidant effects of oleuropein as a natural antioxidant agent on intestine mucosal damages induced by absolute ethanol were investigated in the present study. The 42 adult male rats were divided into four (control, oleuropein, ethanol, and oleuropein plus ethanol) groups. Oleuropein at a dosage of 12 mg/kg was used for 10 consecutive days in oleuropein and oleuropein plus ethanol groups, thereafter absolute ethanol (once, 1 ml/rat) was administrated orally in ethanol and oleuropein plus ethanol groups at fasting state by gavage. The duodena of rats were removed for histopathology and antioxidant assay. Histological evidence of severe mucosal damages were confirmed by histopathology findings in ethanol group and prevented in oleuropein plus ethanol group. In this setting, glutathione peroxidase and catalase activities were significantly higher in oleuropein and oleuropein plus ethanol groups than the ethanol-treated rats. In contrast, thiobarbituric acid reactive substances concentration (as a lipid peroxidation marker) significantly increased in ethanol-treated rats when compared to the other groups. Our results suggest that olive leaf extract (containing oleuropein) exerts a potent antioxidant agent against oxidative stress. These findings allow us to exploit the advantages of oleuropein treatment in various diseases induced by oxidative stress in humans. © 2013, Springer-Verlag London.

引用

页码：1359 / 1365

页数：6

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