Sprout differentiation and mutation induction of garlic (Allium sativum L.) callus exposed to gamma radiation

被引:0
作者
Hassan H. A. Mostafa
Haiping Wang
Di Shen
Yang Qiu
Xixiang Li
机构
[1] Chinese Academy of Agricultural Sciences,Beijing Research Station for Vegetable Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture, Institute of Vegetables and Flowers
[2] Agricultural Research Centre,Central Laboratory of Organic Agriculture
来源
Plant Growth Regulation | 2015年 / 75卷
关键词
Calli; Bud differentiation; Gamma radiation; Mutation;
D O I
暂无
中图分类号
学科分类号
摘要
Germplasm enhancement and breeding is difficult for garlic (Allium sativum L.) as it can only be vegetatively propagated. Hence, mutation induction is still the most effective way to create new varieties. Calli from two Chinese commercial garlic varieties, Zhoumou (ZM) and Yongnian (YN), were treated with different dosages (1, 3, 5 and 7 Gy) of gamma (γ) radiation. The results showed that the two genotypes differed in their sensitivity to γ-radiation. YN was sensitive to high dosage, while ZM showed better growth at higher dosage. More specifically, the average number of calli producing sprouts, sprout number per callus, total sprouts number, the number of sprouts forming plantlets and plantlet lengths were higher when calli of variety YN were treated with 1 Gy. However, the corresponding parameters in ZM were higher for 7 Gy. Simple sequence repeat analysis with two of 16 novel primers showed genetic variation among the plantlets following γ-radiation treatments of 5 Gy for variety YN and 7 Gy for variety ZM. The methods and mutant materials in this study could be used in future garlic breeding programs.
引用
收藏
页码:465 / 471
页数:6
相关论文
共 114 条
  • [41] Javed I(undefined)Effect of 12C+ 5 ion beam irradiation on cell viability and plant regeneration in callus, protoplasts and cell suspensions of undefined undefined undefined-undefined
  • [42] Karthika R(undefined)Effect of gamma rays on different explants of callus treatment of multiple shoots in undefined undefined undefined-undefined
  • [43] Subba LB(undefined) cv. Bathasa undefined undefined undefined-undefined
  • [44] Khai TH(undefined)Effects of gamma irradiation on morphological changes and biological responses in plants undefined undefined undefined-undefined
  • [45] Lang NT(undefined)In vitro mutagenesis and identification of mutants via ISSR in lily ( undefined undefined undefined-undefined
  • [46] Kim JH(undefined)) undefined undefined undefined-undefined
  • [47] Baek MH(undefined)A primary study on mutation induction of in vitro cultured garlic shoots by undefined undefined undefined-undefined
  • [48] Chung BY(undefined)Co γ-ray irradiation undefined undefined undefined-undefined
  • [49] Wi SG(undefined)Effects of low doses of short-term gamma irradiation on growth and development through two generations of undefined undefined undefined-undefined
  • [50] Kim JS(undefined)undefined undefined undefined undefined-undefined