Development of EST-based new SSR markers in seabuckthorn

被引：21

作者：

Jain A. ^{[1
]}

Ghangal R. ^{[1
]}

Grover A. ^{[1
,3
]}

Raghuvanshi S. ^{[2
]}

Sharma P.C. ^{[1
]}

机构：

[1] University School of Biotechnology, Guru Gobind Singh Indraprastha University, Delhi 110403, Kashmere Gate

[2] Interdisciplinary Center for Plant Genomics, Department of Plant Molecular Biology, University of Delhi, New Delhi 110021, South Campus, Benito Juarez Marg

[3] Defense Institute of Bio-Energy Research, Haldwani 263139, Goraparao

来源：

Physiology and Molecular Biology of Plants | 2010年 / 16卷 / 4期

关键词：

Cross-amplification; Expressed sequence tags; Hippophae rhamnoides; Simple sequence repeats (SSRs);

D O I：

10.1007/s12298-010-0037-3

中图分类号：

学科分类号：

摘要：

EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics. © 2010 Prof. H.S. Srivastava Foundation for Science and Society.

引用

页码：375 / 378

页数：3

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